ACTIVATION OF THE HEAT-STABLE POLYPEPTIDE OF THE ATP-DEPENDENT PROTEOLYTIC SYSTEM
ACTIVATION OF THE HEAT-STABLE POLYPEPTIDE OF THE ATP-DEPENDENT PROTEOLYTIC SYSTEM
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DOI：
10.1073/pnas.78.2.761
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
HERSHKO, A
中科院分区：
文献类型：
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作者：
CIECHANOVER, A;HELLER, H;HERSHKO, A
[兔]网织红细胞的ATP依赖性蛋白水解系统的热稳定多肽，命名为APF-1，在ATP需要过程中与蛋白质底物形成共价结合物。描述了一种通过ATP活化具有焦磷酸盐置换的多肽的酶。AMP-多肽的形成和多肽向二级受体的转移分别由APF-1对ATP-PPi和ATP-AMP交换反应的要求提出。与放射性标记的多肽，ATP依赖性标记的酶被证明是由一个连接，是酸稳定的，但不稳定的处理与温和的碱，羟胺，硼氢化物或汞盐。AMP-多肽显然受到酶的-SH基团的攻击，形成硫酯。
The heat-stable polypeptide of the ATP-dependent proteolytic system of [rabbit] reticulocytes, designated APF-1, forms covalent conjugates with protein substrates in an ATP-requiring process. An enzyme that carries out the activation by ATP of the polypeptide with pyrophosphate displacement is described. The formation of AMP-polypeptide and transfer of the polypeptide to a secondary acceptor are suggested by an APF-1 requirement for ATP-PPi and ATP-AMP exchange reactions, respectively. With radiolabeled polypeptide, an ATP-dependent labeling of the enzyme was shown to be by a linkage that is acid stable but is labile to treatment with mild alkali, hydroxylamine, borohydride or mercuric salts. The AMP-polypeptide apparently undergoes attack by an -SH group of the enzyme to form a thiolester.