Rapid method for detecting resistance to a Qol fungicide in Plasmopara viticola populations
Rapid method for detecting resistance to a Qol fungicide in Plasmopara viticola populations
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DOI：
10.1002/ps.1765
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发表时间：
2009-08-01
影响因子：
4.1
通讯作者：
Takayanagi, Tsutomu
中科院分区：
文献类型：
--
作者：
Furuya, Seiichi;Suzuki, Shunji;Takayanagi, Tsutomu
背景：Qo抑制剂(Qol)-抗杀菌剂葡萄浆原菌(Plasmopara viticola, Berk。& MA柯蒂斯)伯尔。在世界范围内，葡萄霜霉病的防治已成为一个严重的问题。结果：采用巢式PCR-RFLP方法，建立了一种快速检测葡萄葡萄种群对一种Qol杀菌剂氮唑菌酯抗性的方法。利用该方法，在日本葡萄居群细胞色素b基因密码子143处发现了甘氨酸与丙氨酸的替换。结论：巢式PCR-RFLP方法是一种快速、灵敏、可靠的检测偶氮氧嘧啶耐药葡萄假单胞菌群体的方法。(C) 2009年化学工业学会
BACKGROUND: The increasing occurrence of Qo inhibitor (Qol)-fungicide-resistant Plasmopara viticola (Berk. & MA Curtis) Berl. & DeToni populations is becoming a serious problem in the control of grapevine downy mildew worldwide.RESULTS: The authors have developed a rapid method for detecting resistance to a Qol fungicide, azoxystrobin, in P. viticola populations using the nested PCR-RFLP method. With this method, a glycine-to-alanine substitution was discovered at codon 143 in the cytochrome b gene of P. viticola populations found in Japan.CONCLUSION: It is proposed that the nested PCR-RFLP method is a high-speed, sensitive and reliable tool for detecting azoxystrobin-resistant P. viticola populations. (C) 2009 Society of Chemical Industry