Positive association between serum thymic stromal lymphopoietin and anti-citrullinated peptide antibodies in patients with rheumatoid arthritis.
Positive association between serum thymic stromal lymphopoietin and anti-citrullinated peptide antibodies in patients with rheumatoid arthritis.
复制标题
类风湿关节炎患者血清胸腺基质淋巴细胞生成素与抗瓜氨酸肽抗体呈正相关。
DOI：
10.1111/cei.12632
复制
发表时间：
2015
期刊：
影响因子：
4.6
通讯作者：
Haro H, Nakao A
中科院分区：
文献类型：
--
作者：
Koyama K;Ohba T;Haro H, Nakao A
胸腺基质淋巴细胞生成素（TSLP）在类风湿关节炎（RA）的病理生理中起重要作用。然而，关于RA患者血清TSLP浓度及其临床意义的资料很少。本研究调查了RA患者的血清TSLP浓度是否受到影响。采用酶联免疫吸附试验（ELISA）检测了100例RA患者、60例骨关节炎（OA）患者和34例健康志愿者血清中TSLP的浓度。我们还研究了血清TSLP浓度与RA疾病活动性的临床参数[使用28个关节计数的疾病活动性评分（DAS 28）-C-反应蛋白（CRP）、DAS 28-红细胞沉降率（ESR）、临床疾病活动指数（CDAI）、患者/医生的视觉疼痛评分（VAS）、肿胀关节计数、压痛关节计数、CRP、ESR和基质金属蛋白酶-3（MMP-3）浓度]。此外，我们还研究了血清TSLP浓度与抗瓜氨酸肽抗体（ACPA）和血清肿瘤坏死因子（TNF）-α的相关性。RA患者血清TSLP水平显著高于OA患者和健康志愿者。有趣的是，血清TSLP浓度与ACPA滴度显著相关，但与其他临床参数无关。RA患者血清TSLP浓度显著升高，且与ACPA滴度呈正相关。这些发现表明，在RA患者中，TSLP可能在B细胞产生ACPA中发挥作用。
Thymic stromal lymphopoietin (TSLP) has been suggested recently to play an important role in the pathophysiology of rheumatoid arthritis (RA). However, there is little information on serum TSLP concentrations in RA and its clinical significance. The present study investigated whether serum TSLP concentrations were affected in patients with RA. Using an enzyme-linked immunosorbent assay (ELISA), we measured TSLP concentrations in the serum obtained from 100 patients with RA, 60 patients with osteoarthritis (OA) and 34 healthy volunteers. We also investigated the correlation between serum TSLP concentrations and clinical parameters of disease activity in RA [disease activity score using 28 joint counts (DAS28)-C-reactive protein (CRP), DAS28-erythrocyte sedimentation rate (ESR), Clinical Disease Activity Index (CDAI]), patient's/-physician's Visual Analogue Scale (VAS), swollen joints count, tender joints count, CRP, ESR and matrix metalloproteinase-3 (MMP-3) concentrations]. In addition, we investigated the correlation between serum TSLP concentrations and anti-citrullinated peptide antibody (ACPA) and serum tumour necrosis factor (TNF)-α. Serum TSLP levels in patients with RA were significantly higher than those in patients with OA and in healthy volunteers. Interestingly, serum TSLP concentrations were correlated significantly with ACPA titres, but not with other clinical parameters. There was a significant increase in serum TSLP concentrations in patients with RA, which was correlated positively with serum ACPA titres. These findings suggest that in patients with RA, TSLP may play a role in ACPA production by B cells.