Maintenance of cell type-specific connectivity and circuit function requires Tao kinase
Maintenance of cell type-specific connectivity and circuit function requires Tao kinase
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DOI：
10.1038/s41467-019-11408-1
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发表时间：
2019-07
影响因子：
16.6
通讯作者：
Federico Tenedini;Maria Sáez González;Chun Hu;Lisa H. Pedersen;Mabel Matamala Petruzzi;Bettina Spitzweck;Denan Wang;Melanie Richter;Meike Petersen;E. Szpotowicz;M. Schweizer;S. Sigrist;Froylan Calderón de Anda;P. Soba
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文献类型：
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作者：
Federico Tenedini;Maria Sáez González;Chun Hu;Lisa H. Pedersen;Mabel Matamala Petruzzi;Bettina Spitzweck;Denan Wang;Melanie Richter;Meike Petersen;E. Szpotowicz;M. Schweizer;S. Sigrist;Froylan Calderón de Anda;P. Soba
感觉回路通常在发育早期建立，但回路的特异性和功能在机体生长过程中是如何维持的尚未阐明。为了深入了解，我们定量研究了幼虫发育过程中果蝇感觉网络的突触生长和连通性。我们发现初级伤害感受器与其下游神经元之间的连通性与动物的大小有关。我们进一步确定了保守的ste20样激酶Tao作为维持回路特异性和连通性所需的突触生长的负调节因子。在幼体生长过程中，Tao激酶的缺失导致幼体突触后特化和异常连接。通过功能成像和行为分析，我们发现陶氏诱导的异位突触与不合适的伙伴神经元的缺失是功能性的，并以连接特异性的方式改变行为反应。我们的数据表明，在动物生长过程中，需要Tao激酶对突触生长进行微调，以维持神经元网络的特异性和行为输出。
Sensory circuits are typically established during early development, yet how circuit specificity and function are maintained during organismal growth has not been elucidated. To gain insight we quantitatively investigated synaptic growth and connectivity in theDrosophilanociceptive network during larval development. We show that connectivity between primary nociceptors and their downstream neurons scales with animal size. We further identified the conserved Ste20-like kinase Tao as a negative regulator of synaptic growth required for maintenance of circuit specificity and connectivity. Loss of Tao kinase resulted in exuberant postsynaptic specializations and aberrant connectivity during larval growth. Using functional imaging and behavioral analysis we show that loss of Tao-induced ectopic synapses with inappropriate partner neurons are functional and alter behavioral responses in a connection-specific manner. Our data show that fine-tuning of synaptic growth by Tao kinase is required for maintaining specificity and behavioral output of the neuronal network during animal growth.