Atrial natriuretic factor receptors in rat kidney, adrenal gland, and brain: autoradiographic localization and fluid balance dependent changes.
Atrial natriuretic factor receptors in rat kidney, adrenal gland, and brain: autoradiographic localization and fluid balance dependent changes.
复制标题
大鼠肾脏、肾上腺和大脑中的心房钠尿因子受体：放射自显影定位和液体平衡依赖性变化。
DOI：
10.1073/pnas.83.10.3557
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发表时间：
1986
影响因子：
11.1
通讯作者：
Snyder,SH
中科院分区：
文献类型：
--
作者：
Lynch,DR;Braas,KM;Snyder,SH
哺乳动物心房含有称为心房钠尿因子（ANF）的钠尿肽。使用 125 I 标记的 ANF 进行体外放射自显影，我们定位了肾小球、肾上腺肾小球带和脑脉络丛的高亲和力 (Kd= 150 pM) ANF 结合位点。缺水的大鼠肾脏和肾上腺部位的数量均增加；肾脏缺水 72 小时内和肾上腺 24 小时内可检测到增加。给予 2.0% NaCl 作为饮用水的大鼠和糖尿病大鼠的受体数量下降。这些受体的离散定位和动态变化表明，ANF 通过对肾脏、肾上腺和大脑等众多器官的受体产生多种但协调的影响来调节液体平衡。
Mammalian atria contain natriuretic peptides designated atrial natriuretic factors (ANF). Usingin vitroautoradiography with125I-labeled ANF, we have localized high-affinity (Kd= 150 pM) ANF binding sites to the glomeruli of the kidney, zona glomerulosa of the adrenal gland, and choroid plexus of the brain. The numbers of sites in both kidney and adrenal are increased in rats deprived of water; increases are detectable within 72 hr of water deprivation in the kidney and within 24 hr in the adrenal gland. Receptor numbers decline in rats given 2.0% NaCl as drinking water and in diabetic rats. The discrete localizations and dynamic alterations of these receptors suggest that ANF regulates fluid balance through diverse but coordinated effects on receptors in numerous organs including the kidney, adrenal, and brain.