Convergent responses to stress.: Solar ultraviolet-B radiation and Manduca sexta herbivory elicit overlapping transcriptional responses in field-grown plants of Nicotiana longiflora
Convergent responses to stress.: Solar ultraviolet-B radiation and Manduca sexta herbivory elicit overlapping transcriptional responses in field-grown plants of Nicotiana longiflora
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DOI：
10.1104/pp.103.024323
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发表时间：
2003-08-01
期刊：
影响因子：
7.4
通讯作者：
Ballaré, CL
中科院分区：
文献类型：
--
作者：
Izaguirre, MM;Scopel, AL;Ballaré, CL
过去 20 年来，结合平流层臭氧消耗的生物影响研究，人们对太阳紫外线 (UV)-B（280-315 nm）对植物的影响进行了深入研究。然而，介导植物对太阳（环境）UV-B 反应的分子机制及其与其他应激源激活的反应机制的相互作用在很大程度上仍不清楚。使用富含伤口和昆虫响应序列的微阵列，我们检测了田间种植的长花烟草植物中 241 个基因对环境 UV-B 的表达响应。阵列上大约 20% 的序列显示出对太阳 UV-B 响应的差异表达。对 UV-B 的表达反应与模拟天蛾食草动物引起的表达反应相似。最明显的相似之处是：（a）几个光合作用相关基因的下调，以及（b）参与脂肪酸代谢和氧脂素生物合成的基因上调，例如HPL（氢过氧化物裂合酶）、α-DIOX（α-双加氧酶）、LOX（13-脂氧合酶）和AOS（丙二烯氧化物合酶）。编码 WRKY 转录因子、铁氧还蛋白依赖性谷氨酸合酶的基因以及其他几种功能未知的昆虫反应基因也同样受到 UV-B 和昆虫食草处理的调节。我们的研究结果表明，UV-B 和毛毛虫食草动物激活了共同的调控元件，并为了解 UV-B 对昆虫食草动物的影响机制提供了一个平台，这些机制已在最近的实地研究中记录下来。
The effects of solar ultraviolet (UV)-B (280-315 nm) on plants have been studied intensively over the last 2 decades in connection with research on the biological impacts of stratospheric ozone depletion. However, the molecular mechanisms that mediate plant responses to solar (ambient) UV-B and their interactions with response mechanisms activated by other stressors remain for the most part unclear. Using a microarray enriched in wound- and insect-responsive sequences, we examined expression responses of 241 genes to ambient UV-B in field-grown plants of Nicotiana longiflora Cav. Approximately 20% of the sequences represented on the array showed differential expression in response to solar UV-B. The expression responses to UV-B had parallels with those elicited by simulated Manduca sexta herbivory. The most obvious similarities were: (a) down-regulation of several photosynthesis-related genes, and (b) up-regulation of genes involved in fatty acid metabolism and oxylipin biosynthesis such as HPL (hydroperoxide lyase), alpha-DIOX (alpha-dioxygenase), LOX (13-lipoxygenase), and AOS (allene oxide synthase). Genes encoding a WRKY transcription factor, a ferredoxin-dependent glutamate-synthase, and several other insect-responsive genes of unknown function were also similarly regulated by UV-B and insect herbivory treatments. Our results suggest that UV-B and caterpillar herbivory activate common regulatory elements and provide a platform for understanding the mechanisms of UV-B impacts on insect herbivory that have been documented in recent field studies.