GROWTH OF NITROBACTER BY DISSIMILATORIC NITRATE REDUCTION
GROWTH OF NITROBACTER BY DISSIMILATORIC NITRATE REDUCTION
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DOI：
10.1111/j.1574-6968.1987.tb02524.x
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发表时间：
1987-12-01
影响因子：
2.1
通讯作者：
BOCK, E
中科院分区：
文献类型：
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作者：
FREITAG, A;RUDERT, M;BOCK, E
8株硝化细菌在硝酸盐存在的厌氧条件下生长。浓度在0.5 mM以上的亚硝酸盐对其生长有抑制作用，通过特殊的培养技术可以消除亚硝酸盐对其生长的抑制作用。亚硝酸盐氧化细胞在气密培养瓶中作为生物膜生长在透气性硅胶管上。生物膜允许硝酸盐还原细胞在低亚硝酸盐浓度下生长。这些细胞要么在厌氧培养基中活跃地生长，要么在生物膜的厌氧区生长。它们产生了亚硝酸盐和氨。氮平衡计算表明，8株菌株中有5株存在无机氮损失。这意味着硝酸还原细胞进一步产生挥发性氮化合物。气相色谱法检测N2O。
Eight strains of the genusNitrobactergrew under anaerobic conditions in the presence of nitrate. The growth was inhibited by nitrite concentrations above 0.5 mM. By a special culture technique inhibition caused by nitrite was abolished. Nitrite oxidizing cells grew in gas tight culture flasks as a biofilm on a gas-permeable silicone tubing. The biofilm allowed nitrate-reducing cells to grow at a low nitrite concentration. These cells grew either actively motile in the anaerobic medium, or in anaerobic zones of the biofilm. They produced nitrite and ammonia. Nitrogen balance calculations established a loss of inorganic nitrogen for 5 of 8 strains. This implies that nitrate-reducing cells produced furthermore volatile nitrogen compounds. N2O was detected by gas chromatography.