A PCB-based electronic ELISA system for rapid, portable infectious disease diagnosis
A PCB-based electronic ELISA system for rapid, portable infectious disease diagnosis
复制标题
DOI：
10.1109/biocas.2016.7833779
复制
发表时间：
2016-10
期刊：
影响因子：
--
通讯作者：
K. Papadimitriou;Daniel Evans;H. Morgan;T. Prodromakis
中科院分区：
文献类型：
--
作者：
K. Papadimitriou;Daniel Evans;H. Morgan;T. Prodromakis
本文提出了一种基于专用印刷电路板(PCB)技术的电流型电化学检测方法。设计并制作了一块便携式、可重构的多通道安培数据采集板，专门用于测量基于印刷电路板的生物传感器提供的电流输入信号。电子读出电路能够为安培传感器提供恒定的偏置电压，实时测量传感器的输出电流，使用高精度模数转换器(ADC)将其数字化，并通过USB2.0接口或板载TFT触摸屏将二进制数据发送给用户。为了验证组合系统的稳健性和准确性，我们使用我们定制的基于印刷电路板的系统和标准的电化学衬底进行了概念验证安培实验。通过标准比色分析对所得结果进行了交叉验证，并突出和分析了它们之间的差异。
In this paper an amperometric electrochemical detection method is demonstrated and implemented using exclusively Printed Circuit Board (PCB)-based technologies. A portable, reconfigurable, multichannel amperometric data-acquisition board has been designed and fabricated, dedicated to the measurement of current-input signals delivered by the PCB-based biosensor. The electronic read-out circuit is able to provide constant biasing voltages to the amperometric sensor, measure in real-time the sensor's output currents, digitise them using high-accuracy Analog-to-Digital Converters (ADCs) and send the binary data to the user either through a USB2.0 interface or via an on-board TFT touch-screen. In order to validate the robustness and accuracy of the combined system, proof-of-concept amperometric experiments have taken place using our custom-made PCB-based system and standard electrochemical substrates. The results obtained have been cross-validated by means of standard colorimetric analysis and their differences have been highlighted and analyzed.