Identification of One Novel Candidate Probiotic Lactobacillus plantarum Strain Active against Influenza Virus Infection in Mice by a Large-Scale Screening
Identification of One Novel Candidate Probiotic Lactobacillus plantarum Strain Active against Influenza Virus Infection in Mice by a Large-Scale Screening
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DOI：
10.1128/aem.03075-12
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发表时间：
2013-03-01
影响因子：
4.4
通讯作者：
Bermudez-Humaran, Luis G.
中科院分区：
文献类型：
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作者：
Kechaou, Noura;Chain, Florian;Bermudez-Humaran, Luis G.
在这项研究中，我们开展了大规模的细菌菌株筛选，以确定具有免疫调节特性的新型候选益生菌。为此，158个菌株，包括大多数乳酸菌(LAB)，通过两种不同的细胞模型进行筛选：肿瘤坏死因子α(TNF-α)激活的HT-29细胞和外周血单核细胞(PBMC)。选择了对两种模型都有反应的不同菌株(促炎和抗炎菌株)，并在小鼠流感病毒感染模型中测试了它们的保护作用。植物乳杆菌CNRZ1997是一种潜在的促炎益生菌，在病毒攻击(100pfu的H1N1流感病毒A波多黎各/8/1934[A/PR8/34]/小鼠)前10天和之后10天，每天灌胃给药，通过减少体重减轻、缓解临床症状和显著抑制病毒在肺部的增殖，显著改善小鼠的健康状况。总之，在这项研究中，我们结合了两个细胞模型，以允许筛选大量的LAB，以了解它们的免疫调节特性。此外，我们还鉴定了一个新的候选益生菌菌株，植物乳杆菌CNRZ1997，它对小鼠的流感病毒感染具有活性。
In this study, we developed a large-scale screening of bacterial strains in order to identify novel candidate probiotics with immunomodulatory properties. For this, 158 strains, including a majority of lactic acid bacteria (LAB), were screened by two different cellular models: tumor necrosis factor alpha (TNF-alpha)-activated HT-29 cells and peripheral blood mononuclear cells (PBMCs). Different strains responsive to both models (pro- and anti-inflammatory strains) were selected, and their protective effects were tested in vivo in a murine model of influenza virus infection. Daily intragastric administrations during 10 days before and 10 days after viral challenge (100 PFU of influenza virus H1N1 strain A Puerto Rico/8/1934 [A/PR8/34]/mouse) of Lactobacillus plantarum CNRZ1997, one potentially proinflammatory probiotic strain, led to a significant improvement in mouse health by reducing weight loss, alleviating clinical symptoms, and inhibiting significantly virus proliferation in lungs. In conclusion, in this study, we have combined two cellular models to allow the screening of a large number of LAB for their immunomodulatory properties. Moreover, we identified a novel candidate probiotic strain, L. plantarum CNRZ1997, active against influenza virus infection in mice.