INVESTIGATION OF CHROMIUM, CERIUM AND COBALT AS MARKERS IN DIGESTA - RATE OF PASSAGE STUDIES
INVESTIGATION OF CHROMIUM, CERIUM AND COBALT AS MARKERS IN DIGESTA - RATE OF PASSAGE STUDIES
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DOI：
10.1002/jsfa.2740310702
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发表时间：
1980-01-01
影响因子：
4.1
通讯作者：
VANSOEST, PJ
中科院分区：
文献类型：
--
作者：
UDEN, P;COLUCCI, PE;VANSOEST, PJ
研究了一种新的液体标记物——钴-乙二胺四乙酸 (EDTA) 和两种固体标记物——Cr 和 Ce 媒染植物细胞壁。描述了标记物的合成和分析方法。测试了 Cr 和 Ce 细胞壁复合物对 EDTA、HCl 和瘤胃微生物的稳定性。植物细胞壁通过 Cr 媒染而变得不可消化，在模拟消化序列（体外）后，98% 的标记物仍保留在纤维上。使用瘤胃培养物，Ce-媒染的细胞壁在体外可消化 35%，并且 56% 的标记物可以从剩余纤维上洗掉。用 EDTA 处理去除了所有 Ce 和 15% 的 Cr。 HCl (0.01 M) 对去除细胞壁上的 Cr 的影响可以忽略不计，而 0.1 M 酸平均去除 10% 的标记物。媒染剂中 Cr 的损失可能与制剂的质量有关。 Co-EDTA 与 Cr-EDTA 相当。大多数动物尿中 Cr 和 Co 的排泄率为 2-3%，但兔子除外，兔子在尿液中排出的液体标记物高达 30%。
A new liquid marker, cobalt-ethylenediamine tetraacetic acid (EDTA) and 2 solid markers, Cr and Ce mordanted plant cell walls, were investigated. Synthesis and methods of analysis are described for the markers. The Cr- and Ce-cell wall complexes were tested for stability to EDTA, HCl and rumen microorganisms. Plant cell walls were rendered indigestible by mordanting with Cr and 98% of the marker remained on the fiber after a simulated sequence (in vitro) of digestion. Ce-mordanted cell walls were 35% digestible in vitro using a rumen culture and 56% of the marker could be washed off the remaining fiber. Treatment with EDTA removed all Ce and 15% of the Cr. HCl (0.01 M) had a negligible effect on the removal of Cr from the cell walls, whereas 0.1 M acid removed, on average, 10% of the marker. Losses of Cr from the mordant may be related to the quality of the preparation. Co-EDTA was comparable to Cr-EDTA. The urinary excretion of Cr and Co was 2-3% in most animals except in rabbits, which excreted as much as 30% of the liquid markers in the urine.