How Single-Cell Technologies Have Provided New Insights Into Atherosclerosis.
How Single-Cell Technologies Have Provided New Insights Into Atherosclerosis.
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DOI：
10.1161/atvbaha.121.315849
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发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Giannarelli C
中科院分区：
文献类型：
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作者：
Eberhardt N;Giannarelli C
创新单细胞技术的发展使得能够对个体血液和组织细胞进行高维转录组学和蛋白质组学分析。最近的单细胞研究揭示了动脉粥样硬化斑块组织的一种新的细胞异质性，并允许更好地了解动脉粥样硬化背景下不同的免疫功能状态。在这篇简短的综述中，我们描述了单细胞技术如何揭示动脉粥样硬化斑块的细胞组成，以及它们对动脉粥样硬化小鼠模型中饮食干扰或遗传操作的反应。我们讨论了scRNA-seq、CITE-seq、ATAC-seq和CyTOF平台如何能够识别动脉粥样硬化进展和消退中的离散免疫、内皮和平滑肌细胞改变。最后，我们回顾了单细胞方法如何允许映射人类动脉粥样硬化斑块的细胞和分子组成，并发现中风患者斑块中新的免疫改变。
The development of innovative single-cell technologies has allowed the high-dimensional transcriptomic and proteomic profiling of individual blood and tissue cells. Recent single-cell studies revealed a new cellular heterogeneity of atherosclerotic plaque tissue and allowed a better understanding of distinct immune functional states in the context of atherosclerosis. In this brief review, we describe how single-cell technologies have shed a new light on the cellular composition of atherosclerotic plaques, and their response to diet perturbations or genetic manipulation in mouse models of atherosclerosis. We discuss how scRNA-seq, CITE-seq, ATAC-seq, and CyTOF platforms have empowered the identification of discrete immune, endothelial, and smooth muscle cells alterations in atherosclerosis progression and regression. Finally, we review how single cell approaches have allowed mapping the cellular and molecular composition of human atherosclerotic plaques and the discovery of new immune alterations in plaques from patients with stroke.