COMPOSITION AND BUFFER CAPACITY OF POOLED STARVED PLAQUE FLUID FROM CARIES-FREE AND CARIES-SUSCEPTIBLE INDIVIDUALS
COMPOSITION AND BUFFER CAPACITY OF POOLED STARVED PLAQUE FLUID FROM CARIES-FREE AND CARIES-SUSCEPTIBLE INDIVIDUALS
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DOI：
10.1177/00220345880670120701
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发表时间：
1988-12-01
影响因子：
7.6
通讯作者：
MORENO, EC
中科院分区：
文献类型：
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作者：
MARGOLIS, HC;DUCKWORTH, JH;MORENO, EC
根据年龄分组的6个人群样本的饥饿斑块液的组成。11日,12.作为。16日,8.作为。17日,18.作为。采用离子色谱法测定患者的龋病状况（无龋，CF, DMFS = 0；易龋，CS, DMFS = 10）。在收集牙菌斑之前，所有受试者48小时不做口腔卫生，并禁食一夜。此外，选定的受试者在收集牙菌斑前一周也接受了牙齿预防。将各组菌斑样本放入矿物油池中，15000 g（室温）离心45分钟。然后分析上清液中的有机酸、无机阴离子、一价和二价阳离子。CF和CS亚组的斑块液样本也用标准盐酸滴定，以探索其固有的缓冲能力。离子Na+ NH4+， K+, CA2+, Mg2+, Cl-，磷酸盐和碳酸盐都存在于所有分析的样品中。检测到甲酸、乙酸、丙酸、丁酸和微量的异丁酸和异戊酸，其中乙酸和丙酸占总酸的85%至94%。空腹斑块液中检测到少量（>.1 mmol/L）至无琥珀酸或乳酸。CF和CS的空腹斑块液的组成和饱和度无明显差异。这些结果表明，龋齿易感性不一定与汇集的饥饿斑块的斑块液组成相关。此外，这些样品的固有缓冲能力没有明显的差异，这与成分的相似性一致。从滴定研究中也可以明显看出，我们的分析中缺少一个重要的缓冲成分；这种缺失的成分似乎是pH值高于6的有效缓冲液。
The composition of pooled starved plaque fluid from six population samples, grouped according to age (8.sbd.11, 12.sbd.16, 8.sbd.17, 18.sbd.22 years) and caries status (caries-free, CF, DMFS = 0; caries-susceptible, CS, DMFS > 10), was determined by means of ion chromatography. Prior to plaque collection, all subjects abstained from oral hygiene for 48 hours and fasted overnight. In addition, selected subjects also received a dental prophylaxis one week prior to plaque collection. Plaque samples from each group were pooled under mineral oil and centrifuged at 15,000 g (ambient temperature) for 45 minutes. Supernatants were then analyzed for organic acids, inorganic anions, and mono- and divalent cations. Plaque fluid samples from CF and from the CS subgroups were also titrated with standard HCl for exploration of the inherent buffer capacity. The ions Na+ NH4+, K+, CA2+, Mg2+, Cl-, phosphate, and carbonate were present in all samples analyzed. Formic, acetic, propionic, butyric, and trace amounts, of isobutyric and isovaleric acids were detected, with acetic and propionic acids comprising between 85 and 94% of the total acids. Little (> 1 mmol/L) to no succinic or lactic acid was detected in starved plaque fluid. No apparent difference was detected in the composition and saturation status of pooled starved plaque fluid from CF and CS individuals. These results suggest that caries susceptibility is not necessarily correlated with the plaque fluid composition of pooled starved plaque. In addition, no apparent difference in the inherent buffer capacities of these samples could be detected, consistent with the noted similarities in composition. It was also apparent from the titration studies that a significant buffering component is missing from our analyses; this missing component appears to be an effective buffer above pH 6.