Synchrotron radiation X-ray diffraction studies on muscle: past, present, and future.
Synchrotron radiation X-ray diffraction studies on muscle: past, present, and future.
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DOI：
10.1007/s12551-019-00554-x
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发表时间：
2019-06-15
影响因子：
--
通讯作者：
Iwamoto, Hiroyuki
中科院分区：
文献类型：
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作者：
Iwamoto, Hiroyuki
X射线衍射是一种以原子尺度的空间分辨率研究材料结构的技术。在生命科学领域，X射线衍射技术特别适用于研究具有周期性结构的材料，如蛋白质晶体、核酸和肌肉。其中，肌肉是一种动态结构，肌肉收缩过程中发生的分子事件一直是肌肉研究者的主要兴趣。在早期，实验室X射线发生器无法提供足够强的X射线通量来解决肌肉中的动态分子事件。随着强同步辐射X射线和先进探测器的出现，这种情况发生了巨大变化，今天可以以亚毫秒的时间分辨率记录肌肉的X射线衍射图案。在这篇综述中，我们主要阐述了肌肉X射线衍射研究的历史和现状的技术方面，并讨论了新技术的进步将使肌肉研究成为可能。
X-ray diffraction is a technique to study the structure of materials at spatial resolutions up to an atomic scale. In the field of life science, the X-ray diffraction technique is especially suited to study materials having periodical structures, such as protein crystals, nucleic acids, and muscle. Among others, muscle is a dynamic structure and the molecular events occurring during muscle contraction have been the main interest among muscle researchers. In early days, the laboratory X-ray generators were unable to deliver X-ray flux strong enough to resolve the dynamic molecular events in muscle. This situation has dramatically been changed by the advent of intense synchrotron radiation X-rays and advanced detectors, and today X-ray diffraction patterns can be recorded from muscle at sub-millisecond time resolutions. In this review, we shed light mainly on the technical aspects of the history and the current status of the X-ray diffraction studies on muscle and discuss what will be made possible for muscle studies by the advance of new techniques.