An ultra-performance liquid chromatography–quadrupole time-of-flight tandem mass spectrometry method based on a four-step analysis strategy to investigate metabolites of Qi-Yu-San-Long decoction in rat plasma
An ultra-performance liquid chromatography–quadrupole time-of-flight tandem mass spectrometry method based on a four-step analysis strategy to investigate metabolites of Qi-Yu-San-Long decoction in rat plasma
复制标题
基于四步分析策略的超高效液相色谱-四极杆时间-飞行串联质谱法研究大鼠血浆中芪、郁、散、龙汤的代谢物
DOI：
10.1002/rcm.9419
复制
发表时间：
2023
影响因子：
2
通讯作者：
Huan Wu
中科院分区：
文献类型：
--
作者：
Yue Zhao;Yang Chen;Ruijuan Li;Ting Zheng;Mengwen Huang;Yating Gao;Zegeng Li;Huan Wu
代谢无疑与中药的疗效和安全性显著相关。临床上，芪愈散龙汤治疗非小细胞肺癌（NSCLC）取得了较好的疗效。然而，对化合物的详细了解QYSLD（原型和代谢产物）及其在血浆中的动态代谢特征尚未揭示。MethodsIn this study，a rapid and sensitive method based on ultra-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry采用UPLC-QTOF/MSE方法，结合四步分析策略，研究QYSLD在大鼠血浆中的代谢特征。在大鼠血浆中鉴定了101种外源性物质（41种原型和60种QYSLD相关代谢物）。研究揭示了QYSLD中生物碱、皂苷、黄酮、环烯醚萜、蒽醌和苯丙素类化合物在大鼠血浆中的代谢特征。在不同的时间间隔，这些外源性物质的动态变化也被观察到。在口服给药后0.5 h，仅检测到15种原型和11种代谢产物。在24 h内，仍能检测到4种原型和20种代谢产物。结论在大鼠血浆中鉴定出QYSLD的101种外源性物质，并系统描述了其代谢动力学特征，为进一步研究QYSLD抑制NSCLC的药效物质奠定了物质基础。
Metabolism is undoubtedly significantly correlated with the efficacy and safety of traditional Chinese medicine. In clinic, Qi‐Yu‐San‐Long decoction (QYSLD) has achieved good results in the treatment of non‐small‐cell lung cancer (NSCLC). Nevertheless, a detailed understanding of the compounds (prototypes and metabolites) of QYSLD and its dynamic metabolic profile in plasma has not been revealed.MethodsIn this study, a rapid and sensitive method based on ultra‐performance liquid chromatography–quadrupole time‐of‐flight tandem mass spectrometry (UPLC–QTOF/MSE), combined with a four‐step analysis strategy, was established to investigate QYSLD metabolic profile in rat plasma.ResultsIn all, 101 xenobiotics (41 prototypes and 60 QYSLD‐related metabolites) were identified in rat plasma. The research uncovered metabolic profiles of alkaloids, saponins, flavonoids, iridoids, anthraquinones, and phenylpropanoids of QYSLD in rat plasma. The dynamic changes in these xenobiotics were also observed at different time intervals. At 0.5 h after oral administration, only 15 prototypes and 11 metabolites were detected. Within 24 h, 4 prototypes and 20 metabolites can still be detected. Four prototypes and 10 metabolites had the phenomenon of emergence–disappearance–reappearancein vivo.ConclusionIn rat plasma, 101 xenobiotics of QYSLD were identified and their dynamic metabolic profiles were systematically delineated, which laid a material basis for further research of the pharmacodynamic substances of QYSLD inhibiting NSCLC.