Absence of surface expression of feline infectious peritonitis virus (FIPV) antigens on infected cells isolated from cats with FIP
Absence of surface expression of feline infectious peritonitis virus (FIPV) antigens on infected cells isolated from cats with FIP
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DOI：
10.1016/j.vetmic.2006.11.026
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发表时间：
2007-03-31
影响因子：
3.3
通讯作者：
Nauwynck, H. J.
中科院分区：
文献类型：
--
作者：
Cornelissen, E.;Dewerchin, H. L.;Nauwynck, H. J.
猫科动物传染性腹膜炎病毒（FIPV）阳性细胞存在于Pyogranulomas中，并且与FIR的猫散发出这些细胞主要属于单核细胞/巨噬细胞谱系。这些细胞如何在免疫猫中存活。在这项研究中，将FIPV阳性细胞从上型瘤中分离出来，并在表面确定了12个自然感染的猫的渗出物，并在其表面确定了两个免疫靶靶猫，病毒抗原和MHC I的存在。大多数感染细胞被证实是单核细胞/巨噬细胞谱系的细胞。在FIPV阳性细胞上未检测到病毒抗原的表面表达。所有FIPV阳性细胞上都存在MHC I分子。在培养了孤立的感染细胞后，在质膜上重新表达了感染细胞的52 +/- 10％的病毒抗原。总而言之，可以说，在FIP猫中，FIPV在单核细胞/巨噬细胞谱系细胞中复制没有在其质膜中携带病毒抗原，这可以使它们摆脱抗体依赖性细胞裂解。 （c）2006 Elsevier B.V.保留所有权利。
Feline infectious peritonitis virus (FIPV) positive cells are present in pyogranulomas and exudates from cats with FIR These cells belong mainly to the monocyte/macrophage lineage. How these cells survive in immune cats is not known. In this study, FIPV positive cells were isolated from pyogranulomas and exudates of 12 naturally FIPV-infected cats and the presence of two immunologic targets, viral antigens and MHC I, on their surface was determined. The majority of the infected cells were confirmed to be cells from the monocyte/macrophage lineage. No surface expression of viral antigens was detected on FIPV positive cells. MHC I molecules were present on all the FIPV positive cells. After cultivation of the isolated infected cells, 52 +/- 10% of the infected cells re-expressed viral antigens on the plasma membrane.In conclusion, it can be stated that in FIP cats, FIPV replicates in cells of the monocyte/macrophage lineage without carrying viral antigens in their plasma membrane, which could allow them to escape from antibody-dependent cell lysis. (c) 2006 Elsevier B.V. All rights reserved.