RNA Phosphorothioate Modification in Prokaryotes and Eukaryotes
RNA Phosphorothioate Modification in Prokaryotes and Eukaryotes
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DOI：
10.1021/acschembio.0c00163
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发表时间：
2020-06-19
影响因子：
4
通讯作者：
Sheng, Jia
中科院分区：
文献类型：
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作者：
Wu, Ying;Tang, Yaning;Sheng, Jia
RNA修饰在RNA结构和基因表达与翻译调控中起着重要作用。我们报告的第一个RNA修饰的磷酸盐，RNA硫代磷酸（PS）修饰，发现在原核生物和真核生物。真核生物核酸的PS修饰也是首次报道。GpsG修饰存在于Rp构型中，并采用液相色谱-串联质谱法（LC-MS/MS）进行定量。通过敲除E. coli中，我们发现调控DNA PS修饰的Dnd簇也可能在RNA PS修饰中发挥作用。我们还发现，在E. coli，L. lactis和HeLa细胞，并且在来自这些细胞的rRNA耗尽的总RNA中未检测到。
RNA modifications play important roles in RNA structures and regulation of gene expression and translation. We report the first RNA modification on the phosphate, the RNA phosphorothioate (PS) modification, discovered in both prokaryotes and eukaryotes. The PS modification is also first reported on nucleic acids of eukaryotes. The GpsG modification exists in the Rp configuration and was quantified with liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). By knocking out the DndA gene in E. coli, we show the Dnd clusters that regulate DNA PS modification may also play roles in RNA PS modification. We also show that the GpsG modification locates on rRNA in E. coli, L. lactis, and HeLa cells, and it is not detected in rRNA-depleted total RNAs from these cells.