Characterization of Fecal Microbial Communities in Patients with Liver Cirrhosis
Characterization of Fecal Microbial Communities in Patients with Liver Cirrhosis
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DOI：
10.1002/hep.24423
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发表时间：
2011-08-01
期刊：
影响因子：
13.5
通讯作者：
Li, Lanjuan
中科院分区：
文献类型：
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作者：
Chen, Yanfei;Yang, Fengling;Li, Lanjuan
肝硬化是慢性肝病的病理终末期。越来越多的证据表明，肠道植物群参与肝硬化并发症的发病机制。本研究的目的是比较肝硬化患者和健康人的粪便微生物群落特征。我们招募了36名肝硬化患者和24名健康对照。通过对16 S核糖体RNA V3区进行454焦磷酸测序，然后进行实时定量聚合酶链反应来分析粪便微生物群落。与健康对照组相比，观察了肝硬化患者粪便微生物群的社区范围变化。拟杆菌门的比例显著降低（P = 0.008），而变形菌门和梭菌门在肝硬化组中高度富集（分别为P = 0.001和0.002）。肠杆菌科（P = 0.001），韦荣球菌科（P = 0.046），链球菌科（P = 0.001）在家庭水平的肝硬化患者中普遍存在。CTP评分与链球菌感染呈正相关（R = 0.386，P = 0.02）。毛螺菌在肝硬化患者中显著减少（P = 0.004），且与CTP评分呈负相关（R =-0.49，P = 0.002）。使用偏最小二乘判别分析，我们确定了149个操作分类单位（OTU）作为肝硬化的关键类型，其中大部分是毛螺菌科（65个OTU），链球菌科（23个OTU）和韦荣球菌科（21个OTU）。结论：肝硬化患者粪便微生物群落与健康人不同。肝硬化患者中潜在致病菌（如肠杆菌科和链球菌科）的流行以及有益菌群（如毛螺菌科）的减少可能影响预后。（肝脏学2011;54：562-572）
Liver cirrhosis is the pathologic end stage of chronic liver disease. Increasing evidence suggests that gut flora is implicated in the pathogenesis of liver cirrhosis complications. The aim of this study was to characterize the fecal microbial communities in patients with liver cirrhosis in comparison with healthy individuals. We recruited 36 patients with liver cirrhosis and 24 healthy controls. The fecal microbial community was analyzed by way of 454 pyrosequencing of the 16S ribosomal RNA V3 region followed by real-time quantitative polymerase chain reaction. Community-wide changes of fecal microbiota in liver cirrhosis were observed compared with healthy controls. The proportion of phylum Bacteroidetes was significantly reduced (P = 0.008), whereas Proteobacteria and Fusobacteria were highly enriched in the cirrhosis group (P = 0.001 and 0.002, respectively). Enterobacteriaceae (P = 0.001), Veillonellaceae (P = 0.046), and Streptococcaceae (P = 0.001) were prevalent in patients with cirrhosis at the family level. A positive correlation was observed between Child-Turcotte-Pugh (CTP) score and Streptococcaceae (R = 0.386, P = 0.02). Lachnospiraceae decreased significantly in patients with cirrhosis (P = 0.004) and correlated negatively with CTP score (R = -0.49, P = 0.002). Using partial least square discriminate analysis, we identified 149 operational taxonomic units (OTUs) as key phylotypes that responded to cirrhosis, most of which were Lachnospiraceae (65 OTUs), Streptococcaceae (23 OTUs), and Veillonellaceae (21 OTUs). Conclusion: Fecal microbial communities are distinct in patients with cirrhosis compared with healthy individuals. The prevalence of potentially pathogenic bacteria, such as Enterobacteriaceae and Streptococcaceae, with the reduction of beneficial populations such as Lachnospiraceae in patients with cirrhosis may affect prognosis. (HEPATOLOGY 2011;54:562-572)