Radioautographic evidence of DNA synthesis in well-differentiated cells of human skin papillomas (verrucae vulgares).
Radioautographic evidence of DNA synthesis in well-differentiated cells of human skin papillomas (verrucae vulgares).
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人类皮肤乳头状瘤（寻常疣）分化良好的细胞中 DNA 合成的放射自显影证据。
DOI：
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发表时间：
1969
影响因子：
6.5
通讯作者：
A. L. Rashad
中科院分区：
文献类型：
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作者：
A. L. Rashad
10例皮肤乳头状瘤和3例正常皮肤的薄组织切片在体外暴露于胸苷-H3 3-5小时，并进行放射自显影。在乳头状瘤中检测到异常的胸苷-H3掺入模式。所有10个肿瘤显示标记在表面分化良好的颗粒细胞的核以及基底细胞和棘细胞。在颗粒层内，标记细胞倾向于出现在病灶中。正常皮肤中的标记仅限于基底层和副基底层细胞。仅在乳头状瘤的基底层和副基底层细胞及表皮中检测到有丝分裂象。基底细胞的比例在肿瘤中为2.6 ~ 13%，在正常表皮中约为4.5%。电镜下观察了10例乳头状瘤中的6例，在所有6例肿瘤中均发现了以前发现的典型形态的病毒颗粒。我们以前曾报道过兔、牛和犬病毒诱导的乳头状瘤颗粒细胞中的DNA合成，但我们没有发现胸苷-H3掺入化学诱导的乳头状瘤颗粒细胞中。现有的证据是一致的概念，即致病性乳头状瘤病毒是负责合成的DNA在分化良好的颗粒细胞。这种新的DNA是病毒DNA，病毒诱导的细胞DNA还是两者都是未知的。然而，似乎没有合理的替代概念，它是病毒指导的DNA合成的结果。异常DNA合成可能是一个有用的标志物，用于检测掩蔽病毒在某些类型的肿瘤，其中分化良好的细胞不能有丝分裂可以确定。
Thin tissue slices from cutaneous papillomas of 10 patients and from normal skin of 3 patients were exposed to thymidine-H 3 in vitro for 3–5 hours, and processed for radio-autography. An abnormal pattern of thymidine-H 3 incorporation was detected in the papillomas. All of the 10 tumors showed label in the nuclei of the superficial well-differentiated granular cells as well as in basal and spiny cells. Within the granular layer there was a tendency for the labeled cells to occur in foci. The label in the normal skin was restricted to the basal and parabasal cells. Mitotic figures were detected only in the basal and parabasal cells of the papillomas and epidermis. The proportion of basal cells labeled varied from 2.6–13 per cent in the tumors; in the norml epidermis it was about 4.5 per cent. Six of the 10 papillomas were examined under the electron microscope and virus particles morphologically typical of those previously found by others were demonstrable in all 6 tumors. We have previously reported DNA synthesis in granular cells of virus-induced papillomas of rabbits, bovines and canines, but we did not find thymidine-H 3 incorporation into granular cells of chemically-induced papillomas. The available evidence is consistent with the concept that the causative papilloma virus is responsible for the synthesis of DNA in the well-differentiated granular cells. Whether this new DNA, is virus DNA, virus-induced cellular DNA or both, is unknown. However, there seems to be no plausible alternative to the concept that it is the result of virus-directed DNA synthesis. Abnormal DNA synthesis may be a useful marker for detecting masked viruses in some types of tumors in which well-differentiated cells incapable of mitotic division can be identified.