VARIABILITY IN RDNA LOCI IN THE GENUS ORYZA DETECTED THROUGH FLUORESCENCE IN-SITU HYBRIDIZATION
VARIABILITY IN RDNA LOCI IN THE GENUS ORYZA DETECTED THROUGH FLUORESCENCE IN-SITU HYBRIDIZATION
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DOI：
10.1007/bf00225782
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发表时间：
1994-03-01
影响因子：
5.4
通讯作者：
KHUSH, GS
中科院分区：
文献类型：
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作者：
FUKUI, K;OHMIDO, N;KHUSH, GS
稻属中的 17s-5.8s-25s 核糖体 RNA 基因 (rDNA) 位点。采用荧光原位杂交（FISH）方法进行鉴定。 rDNA 位点位于 8 个二倍体稻属的 1 至 3 条染色体（2 至 6 个位点）上。 rDNA 位点之一给出最弱的杂交信号。该位点在稻属中是首次报道。含有rDNA位点的染色体按rDNA拷贝数降序确定为第9、10和11号。图像分析方法的应用、载玻片制备处理（后处理）以及热循环仪的使用，极大地提高了结果的再现性。稻属 rDNA 位点变异的进化意义。正在讨论。
The 17s-5.8s-25s ribosomal RNA gene (rDNA) loci in Oryza spp. were identified by the fluorescence in-situ hybridization (FISH) method. The rDNA loci were located on one-to-three chromosomes (two-to-six sites) within the eight diploid Oryza spp. One of the rDNA loci gave the weakest hybridization signal. This locus is reported for the first time in the genus Oryza. The chromosomes containing the rDNA loci were determined to be numbers 9, 10 and 11 in descending order of the copy number of rDNA. The application of image analysis methods, after slide preparation treatments (post-treatments), and the use of a thermal cycler, greatly improved the reproducibility of the results. The evolutionary significance of the variability of rDNA loci among the Oryza spp. is discussed.