The organization of mitochondrial atp6 gene region in male fertile and CMS lines of pepper (Capsicum annuum L.)
The organization of mitochondrial atp6 gene region in male fertile and CMS lines of pepper (Capsicum annuum L.)
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DOI：
10.1007/s00294-005-0032-3
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发表时间：
2006-01-01
期刊：
影响因子：
2.5
通讯作者：
Kim, BD
中科院分区：
文献类型：
--
作者：
Kim, DH;Kim, BD
先前已经比较了雄性可育（N）和CMS（S）辣椒中的线粒体atp 6基因，并发现其以两个拷贝存在（Kim等，J Kor Soc霍特Sci 42：121-127，2001）。在本研究中，通过反向PCR技术扩增这些atp 6拷贝，并对编码区以及5'和3'侧翼区进行测序。在CMS辣椒中检测到atp 6拷贝数为一个完整的基因和一个3'编码区截短的假基因。当将辣椒中的atp 6基因与其他植物物种进行比较时，辣椒、马铃薯和矮牵牛在3'延伸区都具有12个相同的氨基酸序列，这被认为是茄科的标志。北方杂交分析表明，atp 6基因在辣椒细胞质雄性不育系和恢复系中的mRNA带型存在差异，表明atp 6基因是辣椒细胞质雄性不育的候选基因之一。
The mitochondrial atp6 gene in male fertile (N) and CMS (S) pepper has previously been compared and was found to be present in two copies (Kim et al. in J Kor Soc Hort Sci 42:121-127 2001). In the current study, these atp6 copies were amplified by an inverse PCR technique, and the coding region as well as the 5' and 3' flanking regions were sequenced. The atp6 copies in CMS pepper were detected as one intact gene and one pseudogene, truncated at the 3' coding region. When the atp6 genes in pepper were compared to other plant species, pepper, potato, and petunia all possessed a sequence of 12 identical amino acids at the 3' extended region, which was considered a hallmark of the Solanaceae family. Northern blot analysis showed differences in mRNA band patterns between CMS and restorer lines, indicating that atp6 gene is one of the candidates for CMS in pepper.