Expression of the TAL1/SCL transcription factor in physiological and pathological vascular processes.
Expression of the TAL1/SCL transcription factor in physiological and pathological vascular processes.
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TAL1/SCL转录因子在生理和病理血管过程中的表达。
DOI：
10.1002/path.2028
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
Brandt,SJ
中科院分区：
文献类型：
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作者：
Tang,T;Shi,Y;Opalenik,SR;Brantley-Sieders,DM;Chen,J;Davidson,JM;Brandt,SJ
TAL1/SCL转录因子在胚胎发育过程中对造血和血管重塑起着至关重要的作用。为了帮助阐明其在出生后血管形成过程中的作用，我们用免疫细胞化学方法在几种血管形成的实验模型中表征了小鼠Tal1蛋白的表达。在成年小鼠中，Tal1蛋白在罕见的微血管内皮细胞和血管外细胞中表达，从其形态、与血管的接近程度和血管内皮生长因子受体-2的表达来看，暂时被认为是内皮祖细胞。在激素诱导排卵、伤口愈合和肿瘤发展的所有模型中，表达Tal1蛋白的内皮细胞数量都显著增加，但都是一过性的。最后，在荷瘤动物新形成的淋巴管内皮细胞的细胞核中检测到Tal1蛋白。这些结果表明，TAL1在生理性和病理性新生血管的部位由血管内皮细胞和内皮祖细胞表达，提示该转录因子在成人血管生成中发挥作用。这项工作也为TAL1在淋巴管生成中的表达提供了第一个证据。版权所有(C)2006年英国和爱尔兰病理学会。作者：John Wiley&Sons，Ltd.
The TAL1/SCL transcription factor is essential for haematopoietic commitment and vascular remodelling during embryonic development. To help clarify its role in postnatal vascular processes, we characterized the expression of mouse Tal1 protein by immunocytochemistry in several experimental models of blood vessel formation. In adult mice, Tal1 protein was expressed in rare microvascular endothelial cells and in extravascular cells provisionally identified as endothelial progenitors from their morphology, proximity to vessels and expression of vascular endothelial growth factor receptor‐2. The number of Tal1‐expressing endothelial cells increased significantly but transiently in all the models—hormone‐induced ovulation, wound healing and tumour development. Finally, Tal1 protein was detected in the nuclei of newly formed lymphatic endothelial cells in tumour‐bearing animals. These results show that TAL1 is expressed by vascular endothelial cells and endothelial progenitors at sites of physiological and pathological neovascularization and suggest a role for this transcription factor in adult vasculogenesis. This work also provides the first evidence for TAL1 expression in lymphangiogenesis. Copyright © 2006 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.