Metabolite profiling of the moss Physcomitrella patens reveals evolutionary conservation of osmoprotective substances
Metabolite profiling of the moss Physcomitrella patens reveals evolutionary conservation of osmoprotective substances
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DOI：
10.1007/s00299-011-1177-9
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发表时间：
2012-02-01
影响因子：
6.2
通讯作者：
Reski, Ralf
中科院分区：
文献类型：
--
作者：
Erxleben, Anika;Gessler, Arthur;Reski, Ralf
藓类小立碗藓适合系统生物学研究，因为它可以在标准化条件下在普通矿物培养基中无菌生长，并且仅包含少数细胞类型。我们报告的两个主要的P. patens组织，丝状原丝体和叶状配子体，从不同的培养条件下的代谢产物谱。共检测到96种化合物，其中21种在公共数据库中未知。原丝体和配子体具有不同的代谢特征，特别是在糖类、糖衍生物、氨基酸、木质素前体和富氮储存化合物方面。建立了垂序商陆水培体系，并在生理条件下进行了干旱胁迫试验。这种处理导致了植物保护剂的积累，如阿卓糖、麦芽糖醇、抗坏血酸和脯氨酸。因此，这些植物保护剂并不是种子植物所独有的，而是在植物对土地定殖的早期阶段进化而来的。
The moss Physcomitrella patens is suitable for systems biology studies, as it can be grown axenically under standardised conditions in plain mineral medium and comprises only few cell types. We report on metabolite profiling of two major P. patens tissues, filamentous protonema and leafy gametophores, from different culture conditions. A total of 96 compounds were detected, 21 of them as yet unknown in public databases. Protonema and gametophores had distinct metabolic profiles, especially with regard to saccharides, sugar derivates, amino acids, lignin precursors and nitrogen-rich storage compounds. A hydroponic culture was established for P. patens, and was used to apply drought stress under physiological conditions. This treatment led to accumulation of osmoprotectants, such as altrose, maltitol, ascorbic acid and proline. Thus, these osmoprotectants are not unique to seed plants but have evolved at an early phase of the colonization of land by plants.