VS38 staining contributes to a novel gating strategy in flow cytometry for small B cell lymphoma, especially in lymphoplasmacytic lymphoma/Waldenstrom macroglobulinemia
VS38 staining contributes to a novel gating strategy in flow cytometry for small B cell lymphoma, especially in lymphoplasmacytic lymphoma/Waldenstrom macroglobulinemia
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VS38 染色有助于在流式细胞术中为小 B 细胞淋巴瘤（尤其是淋巴浆细胞淋巴瘤/华氏巨球蛋白血症）提供一种新的门控策略
DOI：
10.1002/cyto.b.22000
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Mitsumasa Watanabe
中科院分区：
文献类型：
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作者：
Shumpei Mizuta;Noriko Yamane;Saya Mononobe;Asami Watanabe;Shinichiro Matsuki;Takao Komai;Yusuke Koba;Sachiko Mitani;Takahito Kawata;Akira Tamekane;Mitsumasa Watanabe
背景多参数流式细胞术（MFC）是检测恶性淋巴瘤肿瘤细胞的有效工具；然而，当特征性免疫表型异常不明显时，淋巴瘤细胞可能难以检测。我们利用MFC评估了用于多发性骨髓瘤的VS38在正常和异常B细胞中的染色性，以开发一种检测淋巴瘤细胞的新策略。方法比较无造血肿瘤患者的淋巴细胞与26例B细胞淋巴瘤（BCL）患者的B细胞VS38染色的中位荧光强度。为了评估VS38门控的性能，我们将VS38阳性B细胞与BCL细胞的百分比进行了比较，并与淋巴浆细胞性淋巴瘤(LPL)/Waldenström巨球蛋白血症（WM）患者中myd88l265p的突变率进行了液滴数字PCR检测。结果scd27阳性记忆B细胞被VS38染色，正常淋巴细胞被微弱染色。12例LPL/WM患者中11例、3例慢性淋巴细胞白血病患者中3例、5例套细胞淋巴瘤患者中3例、4例滤泡性淋巴瘤患者中2例、1例脾边缘带淋巴瘤患者中1例进行了VS38染色。VS38阳性BCL中VS38阳性B细胞的百分比与淋巴瘤细胞的百分比相当，并且myd88l265p在LPL/WM中的突变比率相同。结论svs38在浆细胞疾病和BCL中可识别肿瘤细胞。这可能提高BCL诊断的准确性，特别是在LPL/WM患者中。
BackgroundMulti‐parametric flow cytometry (MFC) is a helpful tool for detecting neoplastic cells in malignant lymphoma; however, lymphoma cells can be difficult to detect when characteristic immunophenotypic abnormalities are not evident. We evaluated the stainability of VS38, which is used for multiple myeloma, in normal and abnormal B cells using MFC to develop a new strategy for detecting lymphoma cells.MethodsWe compared the median fluorescence intensity of VS38 staining in lymphocytes from patients without hematopoietic neoplasms and in B cells from 26 patients with B cell lymphoma (BCL). To evaluate the performance of VS38 gating, we compared VS38‐positive B cells with the percentages of BCL cells, and with the mutation ratios ofMYD88L265P measured by droplet digital PCR in patients with lymphoplasmacytic lymphoma (LPL)/Waldenström macroglobulinemia (WM).ResultsCD27‐positive memory B cells were stained with VS38, whereas normal lymphocytes were faintly stained. Lymphoma cells were stained with VS38 in 11 of 12 patients with LPL/WM, 3 of 3 with chronic lymphocytic leukemia, 3 of 5 with mantle cell lymphoma, 2 of 4 with follicular lymphoma, and 1 of 1 with splenic marginal zone lymphoma. The percentages of VS38‐positive B cells in VS38‐positive BCL were equivalent to those of lymphoma cells and the mutation ratios ofMYD88L265P in LPL/WM.ConclusionsVS38 identified neoplastic cells in plasma cell disorders and BCL. This might improve the accuracy of BCL diagnosis, especially in patients with LPL/WM.