Micromachined flow-through filter-chamber for chemical reactions on beads
Micromachined flow-through filter-chamber for chemical reactions on beads
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DOI：
10.1016/s0925-4005(00)00413-5
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发表时间：
2000-08-10
影响因子：
8.4
通讯作者：
Stemme, G
中科院分区：
文献类型：
--
作者：
Andersson, H;van der Wijngaart, W;Stemme, G
一种新的流通微机械装置上的化学反应珠已被设计，制造和特点。该器件具有简单的平面设计和微加工工艺。磁珠和磁珠都可以收集在反应室中，而不使用外部磁体。液体或气体的样品流通体积是可调节的且不受限制。该装置用Pyrex密封，以允许对化学反应进行真实的实时光学检测。在入口处的恒定压力为3 kPa时，对于所有设计，在过滤室中没有珠粒的情况下，水的流速为约3.5 μ l/min。最小的反应室具有0.5nl的体积，并且可以收集大约50个直径为5.50 μ m的珠。在入口处3 kPa的恒定压力下，当反应室完全填充有珠粒时，水的流速为约2.0 μ l/min。因此，当反应室填充有珠粒时，流速降低约40%。流通式微流体装置对气泡不敏感，并且过滤器的堵塞是罕见且可逆的。珠粒易于从反应室中移除，使得微机械加工的流通装置可重复使用。提出了一种新的、简单的流体互连技术。(C)2000 Elsevier Science B. V.保留所有权利。
A new flow-through micromachined device for chemical reactions on beads has been designed, manufactured, and characterized. The device has an uncomplicated planar design and microfabrication process. Both nonmagnetic and magnetic beads can be collected in the reaction chamber without the use of external magnets. The sample flow-through volume of liquid or gas is adjustable and unlimited. The device is sealed with Pyrex to allow real time optical detection of the chemical reactions. At a constant pressure of 3 kPa at the inlet the flow rate for water is about 3.5 mu l/min without beads in the filter chamber, for all the designs. The smallest reaction chamber has a volume of 0.5 nl and can collect approximately 50 beads with a diameter of 5.50 mu m. At a constant pressure of 3 kPa at the inlet, the flow rate for water is about 2.0 mu l/min when the reaction chamber is completely packed with beads. Hence, the flow rate decreases with about 40% when the reaction chamber is packed with beads. The flow-through microfluidic device is not sensitive to gas bubbles, and clogging of the filter is rare and reversible. The beads are easy to remove from the reaction chamber making the micromachined flow-through device reusable. A new and simple technique for fluid interconnection is developed. (C) 2000 Elsevier Science B.V. All rights reserved.