Biotinylation and isolation of an RNA G-quadruplex based on its peroxidase-mimicking activity
Biotinylation and isolation of an RNA G-quadruplex based on its peroxidase-mimicking activity
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基于其过氧化物酶模拟活性的 RNA G 四链体的生物素化和分离
DOI：
10.1039/c9an00353c
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发表时间：
2019-08-07
期刊：
影响因子：
4.2
通讯作者：
Zhou, Xiang
中科院分区：
文献类型：
--
作者：
Li, Wei;Zeng, Weiwu;Zhou, Xiang
RNA G-四链体（RG 4s）是重要的RNA二级结构，考虑到它们在调节许多细胞过程中的重要性。本文描述了一种rG 4检测和分离方法，其利用rG 4和氯化血红素的复合物来模拟过氧化物酶。在生物素酪酰胺和过氧化氢的存在下，rG 4可以选择性地自我生物素化，并且使用链霉亲和素磁珠容易地从复杂的RNA混合物中分离。
RNA G-quadruplexes (rG4s) are important RNA secondary structures considering their significance in regulating numerous cellular processes. Described herein is an rG4 detecting and isolation method, which exploits the complex of rG4 and hemin to mimic peroxidase. In the presence of biotin tyramide and hydrogen peroxide, rG4s can be selectively self-biotinylated and easily isolated from a complex RNA mixture using streptavidin magnetic beads.