Effects of organic antagonists of Ca(2+), Na(+), and K(+) on chemotaxis and motility of escherichia coli.
Effects of organic antagonists of Ca(2+), Na(+), and K(+) on chemotaxis and motility of escherichia coli.
复制标题
Ca(2)、Na()、K()有机拮抗剂对大肠杆菌趋化性和运动性的影响。
DOI：
10.1128/jb.182.17.4856-4861.2000
复制
发表时间：
2000
影响因子：
3.2
通讯作者：
Adler,J
中科院分区：
文献类型：
--
作者：
Tisa,LS;Sekelsky,JJ;Adler,J
动物研究中使用的各种Ca 2+拮抗剂，其中许多已知是Ca 2+通道阻滞剂，抑制大肠杆菌趋化性（测量为细胞进入含有引诱剂的毛细管）。其中最有效的，在纳摩尔范围内起作用的是ω-芋螺毒素GVIA。其次最有效的是加洛帕米和维拉帕米。在比抑制趋化性所需的浓度高约100倍的浓度下，这些拮抗剂中的每一种都抑制运动性（测量为细胞进入缺乏引诱剂的毛细血管）。其他各种钙离子拮抗剂效果较差，虽然趋化性几乎总是比运动抑制更敏感。用这些Ca 2+拮抗剂中的每一种处理的细胞以运行偏差游动，即，翻滚被抑制。同样地，动物研究中使用的一些Na+拮抗剂抑制细菌趋化性。石房蛤毒素在浓度超过10− 7 M时会抑制大肠杆菌的趋化性，而超过10− 4 M则会抑制大肠杆菌的运动性。用石房蛤毒素处理的细胞以翻滚的偏向游动。在其他Na+拮抗剂的情况下，在动物中，乌头碱抑制细菌的趋化性10倍更有效地抑制运动，和其他两个抑制趋化性和运动在大约相同的浓度。在用于动物研究的K+拮抗剂的情况下，4-氨基吡啶阻断E。在10− 3 M和10− 2 M之间，大肠杆菌的趋化性受到影响，而在10− 2 M时，运动性不受影响;另一方面，氯化四乙基铵在10− 2 M时不能抑制趋化性或运动性。
Various Ca2+antagonists used in animal research, many of them known to be Ca2+channel blockers, inhibitedEscherichia colichemotaxis (measured as entry of cells into a capillary containing attractant). The most effective of these, acting in the nanomolar range, was ω-conotoxin GVIA. The next most effective were gallopamil and verapamil. At concentrations around 100-fold higher than that needed for inhibition of chemotaxis, each of these antagonists inhibited motility (measured as entry of cells into a capillary lacking attractant). Various other Ca2+antagonists were less effective, though chemotaxis was almost always more sensitive to inhibition than was motility. Cells treated with each of these Ca2+antagonists swam with a running bias, i.e., tumbling was inhibited. Similarly, some Na+antagonists used in animal research inhibited bacterial chemotaxis.E. colichemotaxis was inhibited by saxitoxin at concentrations above 10−7M, while more than 10−4M was needed to inhibit motility. Cells treated with saxitoxin swam with a tumbling bias. In the case of other Na+antagonists in animals, aconitine inhibited bacterial chemotaxis 10 times more effectively than it inhibited motility, and two others inhibited chemotaxis and motility at about the same concentration. In the case of K+antagonists used in animal research, 4-aminopyridine blockedE. colichemotaxis between 10−3M and, totally, 10−2M, while motility was not affected at 10−2M; on the other hand, tetraethylammonium chloride failed to inhibit either chemotaxis or motility at 10−2M.