Gradients in pore size enhance the osteogenic differentiation of human mesenchymal stromal cells in three-dimensional scaffolds.
Gradients in pore size enhance the osteogenic differentiation of human mesenchymal stromal cells in three-dimensional scaffolds.
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DOI：
10.1038/srep22898
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发表时间：
2016-03-10
影响因子：
4.6
通讯作者：
Moroni L
中科院分区：
文献类型：
--
作者：
Di Luca A;Ostrowska B;Lorenzo-Moldero I;Lepedda A;Swieszkowski W;Van Blitterswijk C;Moroni L
骨组织中的小骨折可以自行愈合，但在较大缺损的情况下，由于几个缺点，目前的疗法并不完全成功。一种可能的策略依赖于增材制造的聚合物支架和人间充质基质细胞（hMSC）的组合。骨组织的结构以结构梯度为特征。长骨在径向方向上显示结构梯度，而扁骨在轴向方向上显示结构梯度。这种梯度呈现从松质骨到皮质骨的骨密度变化。因此，呈现孔隙率梯度的支架可能是改善骨组织再生的理想候选物。在这项研究中，我们提出了一个结构与离散梯度孔径和表征其能力，以进一步支持hMSCs的成骨分化。此外，我们研究了hMSCs在梯度支架的不同隔室中的行为，显示成骨分化和ECM矿化以及孔尺寸之间的相关性。碱性磷酸酶活性和钙含量随着孔径的增加而增加。我们的结果表明，设计结构孔隙率梯度可能是支持成人干细胞逐渐成骨分化的一种有吸引力的策略。
Small fractures in bone tissue can heal by themselves, but in case of larger defects current therapies are not completely successful due to several drawbacks. A possible strategy relies on the combination of additive manufactured polymeric scaffolds and human mesenchymal stromal cells (hMSCs). The architecture of bone tissue is characterized by a structural gradient. Long bones display a structural gradient in the radial direction, while flat bones in the axial direction. Such gradient presents a variation in bone density from the cancellous bone to the cortical bone. Therefore, scaffolds presenting a gradient in porosity could be ideal candidates to improve bone tissue regeneration. In this study, we present a construct with a discrete gradient in pore size and characterize its ability to further support the osteogenic differentiation of hMSCs. Furthermore, we studied the behaviour of hMSCs within the different compartments of the gradient scaffolds, showing a correlation between osteogenic differentiation and ECM mineralization, and pore dimensions. Alkaline phosphatase activity and calcium content increased with increasing pore dimensions. Our results indicate that designing structural porosity gradients may be an appealing strategy to support gradual osteogenic differentiation of adult stem cells.