LIGHT-SCATTERING MEASUREMENTS SUPPORTING HELICAL STRUCTURES FOR CHROMATIN IN SOLUTION
LIGHT-SCATTERING MEASUREMENTS SUPPORTING HELICAL STRUCTURES FOR CHROMATIN IN SOLUTION
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DOI：
10.1093/nar/5.5.1571
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发表时间：
1978-01-01
影响因子：
14.9
通讯作者：
PARDON, JF
中科院分区：
文献类型：
--
作者：
CAMPBELL, AM;COTTER, RI;PARDON, JF
已对一系列含有 50 至 150 个核小体的多核小体进行了激光散射测量。对于不同离子强度溶液，回转半径已确定为多核小体长度的函数。结果表明，在低离子强度下，染色质采用松散的螺旋结构而不是无规卷曲。随着离子强度的增加，螺旋变得更加规则，其尺寸类似于 Finch 和 Klug 为其螺线管模型提出的尺寸。
Laser light scattering measurements have been made on a series of polynucleosomes containing from 50 to 150 nucleosomes. Radii of gyration have been determined as a function of polynucleosome length for different ionic strength solutions. The results suggest that at low ionic strength the chromatin adopts a loosely helical structure rather than a random coil. The helix becomes more regular on increasing the ionic strength, the dimensions resembling those proposed by Finch and Klug for their solenoid model.