Adsorption Characteristics of Various Proteins to a Titanium Surface
Adsorption Characteristics of Various Proteins to a Titanium Surface
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DOI：
10.1263/jbb.106.273
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发表时间：
2008-09-01
影响因子：
2.8
通讯作者：
Nakanishi, Kazuhiro
中科院分区：
文献类型：
--
作者：
Imamura, Koreyoshi;Shimomura, Miyuki;Nakanishi, Kazuhiro
Adsorption characteristics of 18 proteins, with different sizes and isoelectric points, to a titanium oxide surface were studied. The adsorption isotherms were categorized based on protein type and pH: type 1, irreversible adsorption; type 2, Langmuir-type reversible adsorption; and type 3, reversible and irreversible adsorption. Most of the proteins tested were irreversibly adsorbed in the pH range of 3-8, whereas most adsorbed reversibly at pH 8.5-9.4. Protamine, with a pI value of 12, adsorbed reversibly in the pH range of 3-9. pH values that gave maximal sums of irreversibly and reversibly adsorbed proteins were in the pH range of 3-8 and tended to increase slightly with the pI value of the corresponding protein. pH values that gave maximal quantities of irreversibly adsorbed protein ranged between 4-6 and were nearly independent of pI.
