Retrograde monosynaptic tracing reveals the temporal evolution of inputs onto new neurons in the adult dentate gyrus and olfactory bulb
Retrograde monosynaptic tracing reveals the temporal evolution of inputs onto new neurons in the adult dentate gyrus and olfactory bulb
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DOI：
10.1073/pnas.1218991110
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发表时间：
2013-03-19
影响因子：
11.1
通讯作者：
Berninger, Benedikt
中科院分区：
文献类型：
--
作者：
Deshpande, Aditi;Bergami, Matteo;Berninger, Benedikt
识别成人产生的神经元的连接体对于理解预先存在的回路是如何通过神经发生来完善的至关重要。连接模式的变化可能控制新生成神经元的分化过程，并对其独特的信息处理能力产生重要影响。使用单突触狂犬病病毒为基础的跟踪技术，我们研究了成年产生的神经元在海马和嗅球（OB）的齿状回（DG）在他们的生活的第一个星期的突触前连接的发展。在这两个神经源性区域中，成人产生的神经元首先从多种类型的GABA能中间神经元接收局部连接，然后建立远程投射，例如来自皮质区域的投射。有趣的是，尽管在突触前连接的整体演变模式的基本相似之处，有显着的差异方面的发展皮层的预测：虽然DG颗粒神经元输入起源于内嗅皮层可以追溯到开始只有从3至5周，新产生的神经元在OB接收输入前嗅核和梨状皮质已经由第二周。这种早期的神经递质输入到新产生的中间神经元在OB匹配的时间由同样早期的神经递质苔藓细胞DG颗粒神经元的支配。我们的研究揭示的连接性的发展可能表明将新生成的神经元纳入预先存在的电路的共同原则。
Identifying the connectome of adult-generated neurons is essential for understanding how the preexisting circuitry is refined by neurogenesis. Changes in the pattern of connectivity are likely to control the differentiation process of newly generated neurons and exert an important influence on their unique capacity to contribute to information processing. Using a monosynaptic rabies virus-based tracing technique, we studied the evolving presynaptic connectivity of adult-generated neurons in the dentate gyrus (DG) of the hippocampus and olfactory bulb (OB) during the first weeks of their life. In both neurogenic zones, adult-generated neurons first receive local connections from multiple types of GABAergic interneurons before long-range projections become established, such as those originating from cortical areas. Interestingly, despite fundamental similarities in the overall pattern of evolution of presynaptic connectivity, there were notable differences with regard to the development of cortical projections: although DG granule neuron input originating from the entorhinal cortex could be traced starting only from 3 to 5 wk on, newly generated neurons in the OB received input from the anterior olfactory nucleus and piriform cortex already by the second week. This early glutamatergic input onto newly generated interneurons in the OB was matched in time by the equally early innervations of DG granule neurons by glutamatergic mossy cells. The development of connectivity revealed by our study may suggest common principles for incorporating newly generated neurons into a preexisting circuit.