Individual-specific changes in the human gut microbiota after challenge with enterotoxigenic Escherichia coli and subsequent ciprofloxacin treatment.
Individual-specific changes in the human gut microbiota after challenge with enterotoxigenic Escherichia coli and subsequent ciprofloxacin treatment.
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DOI：
10.1186/s12864-016-2777-0
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发表时间：
2016-06-08
期刊：
影响因子：
4.4
通讯作者：
Stine OC
中科院分区：
文献类型：
--
作者：
Pop M;Paulson JN;Chakraborty S;Astrovskaya I;Lindsay BR;Li S;Bravo HC;Harro C;Parkhill J;Walker AW;Walker RI;Sack DA;Stine OC
产肠毒素大肠杆菌（ETEC）是低收入国家居民和到这些国家旅游的人腹泻的主要原因。人类肠道菌群对ETEC腹泻的发生和发展的影响尚不清楚。我们使用16S rRNA（核糖体RNA）基因测序研究了12名志愿者在ETEC （H10407）和环丙沙星治疗期间粪便微生物群的变化。5名受试者出现严重腹泻，7名受试者很少或没有症状。通过定量培养、定量PCR和16S rRNA基因序列的归一化数检测，腹泻症状与高浓度的粪便大肠杆菌有关。无论是否发生腹泻，微生物群的其他成员在个体之间的变化很大。尽管如此，个体内部的差异与个体之间的差异相比还是很小的。环丙沙星治疗重组微生物群；然而，在1个月和3个月的随访中，原来的结构基本上恢复了。有症状的ETEC感染与粪便中大肠杆菌的高浓度相关，而非无症状感染。感染和环丙沙星治疗都引起其他细菌的变化，通常在三个月后恢复到基线水平。本文的在线版本（doi:10.1186/s12864-016-2777-0）包含补充材料，可供授权用户使用。
Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhea in inhabitants from low-income countries and in visitors to these countries. The impact of the human intestinal microbiota on the initiation and progression of ETEC diarrhea is not yet well understood. We used 16S rRNA (ribosomal RNA) gene sequencing to study changes in the fecal microbiota of 12 volunteers during a human challenge study with ETEC (H10407) and subsequent treatment with ciprofloxacin. Five subjects developed severe diarrhea and seven experienced few or no symptoms. Diarrheal symptoms were associated with high concentrations of fecal E. coli as measured by quantitative culture, quantitative PCR, and normalized number of 16S rRNA gene sequences. Large changes in other members of the microbiota varied greatly from individual to individual, whether or not diarrhea occurred. Nonetheless the variation within an individual was small compared to variation between individuals. Ciprofloxacin treatment reorganized microbiota populations; however, the original structure was largely restored at one and three month follow-up visits. Symptomatic ETEC infections, but not asymptomatic infections, were associated with high fecal concentrations of E. coli. Both infection and ciprofloxacin treatment caused variable changes in other bacteria that generally reverted to baseline levels after three months. The online version of this article (doi:10.1186/s12864-016-2777-0) contains supplementary material, which is available to authorized users.