Filamentous virus-based membrane prepared by chemical cross-linking at liquid/liquid interface for a tailored molecular separation system
Filamentous virus-based membrane prepared by chemical cross-linking at liquid/liquid interface for a tailored molecular separation system
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DOI：
10.1016/j.memsci.2019.117595
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发表时间：
2020-02-01
影响因子：
9.5
通讯作者：
Serizawa, Takeshi
中科院分区：
文献类型：
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作者：
Sawada, Toshiki;Inomata, Haruhiko;Serizawa, Takeshi
具有选择性分离能力的膜在节能过程中是理想的。然而，由于难以控制多孔膜结构和膜组分与靶分子之间的相互作用，精确控制分离的目标选择性仍然具有挑战性。在这项研究中，我们报道了一种基于丝状病毒（M13噬菌体）的膜，通过化学交联在液/液界面制备，用于选择性分子分离。由定向M13噬菌体组合组成的M13噬菌体膜具有分子多孔结构，可实现小分子有机化合物的大小选择性分离。此外，孔结构受M13噬菌体溶液初始浓度控制，具有广泛的尺寸选择性。带正电的分子的排斥率往往大于带负电的分子量相近的分子，这可能与带负电的M13噬菌体膜有关，表明存在一定的静电相互作用。利用对钕离子（Nd3+）具有特异性亲和力的多肽修饰的M13噬菌体制备膜，实现小Nd3+的选择性分离。膜优先吸附Nd3+而不是铁离子，这表明即使在化学交联反应后，肽的分子识别能力仍然明显保持。这种广谱分离膜在废水处理、食品工业和生命科学等领域具有潜在的应用前景。
Membranes with selective separation capability are desirable for use in energy-efficient processes. However, precise control of target selectivity for separation is still challenging due to difficulty in controlling porous membrane structures and interactions between membrane components and target molecules. In this study, we report a filamentous virus (M13 phage)-based membrane prepared by chemical cross-linking at liquid/liquid interfaces for selective molecular separation. The M13 phage membrane composed of oriented M13 phage assemblies showed a molecularly porous structure, enabling the size-selective separation of small organic compounds. Furthermore, the pore structure was controlled by the initial concentration of M13 phage solution, enabling broad size selectivity. The rejection rates of positively charged molecules tend to be greater than those of negatively charged molecules with similar molecular weights, possibly due to the negatively charged M13 phage membrane, indicating certain electrostatic interactions. Membrane preparation using M13 phage modified by peptides with a specific affinity for neodymium ions (Nd3+) was performed to realize the selective separation of small Nd3+. The membrane preferentially adsorbed Nd3+ over iron ions, demonstrating that the molecular recognition capability of the peptide clearly remained even after the chemical cross-linking reaction. This broadspectrum separation membrane has potential applicability in various fields, such as wastewater treatment, the food industry, and the life sciences.