Flaviviral Replication Complex: Coordination between RNA Synthesis and 5'-RNA Capping.
Flaviviral Replication Complex: Coordination between RNA Synthesis and 5'-RNA Capping.
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DOI：
10.3390/v7082837
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发表时间：
2015-08-13
期刊：
影响因子：
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通讯作者：
Choi KH
中科院分区：
文献类型：
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作者：
Klema VJ;Padmanabhan R;Choi KH
黄病毒的基因组复制需要（−）链RNA合成、（+）链RNA合成以及5′-RNA加帽和甲基化。为了进行病毒基因组复制，黄病毒在内质网（ER）膜的细胞质侧组装由病毒和宿主蛋白组成的复制复合物。复制复合物的两个主要组分是病毒非结构（NS）蛋白NS 3和NS 5。它们共同拥有基因组复制所需的所有酶活性，但这些活性在基因组复制过程中如何协调尚不清楚。我们提供了一个概述的黄病毒基因组复制过程中，膜结合复制复合物，和最近的晶体结构全长NS 5。我们提出了一个NS 3和NS 5如何在（−）RNA合成，（+）RNA合成，5′-RNA加帽和甲基化的各个步骤中协调其活动的模型。
Genome replication in flavivirus requires (−) strand RNA synthesis, (+) strand RNA synthesis, and 5′-RNA capping and methylation. To carry out viral genome replication, flavivirus assembles a replication complex, consisting of both viral and host proteins, on the cytoplasmic side of the endoplasmic reticulum (ER) membrane. Two major components of the replication complex are the viral non-structural (NS) proteins NS3 and NS5. Together they possess all the enzymatic activities required for genome replication, yet how these activities are coordinated during genome replication is not clear. We provide an overview of the flaviviral genome replication process, the membrane-bound replication complex, and recent crystal structures of full-length NS5. We propose a model of how NS3 and NS5 coordinate their activities in the individual steps of (−) RNA synthesis, (+) RNA synthesis, and 5′-RNA capping and methylation.