Structure and genomic organization of centromeric repeats in Arabidopsis species
Structure and genomic organization of centromeric repeats in Arabidopsis species
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DOI：
10.1007/s00438-004-1081-x
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发表时间：
2005-02-01
影响因子：
3.1
通讯作者：
Nasuda, S
中科院分区：
文献类型：
--
作者：
Kawabe, A;Nasuda, S
从拟南芥中分离出着丝粒重复序列。gemmifera和A.琴形亚种川崎那。从A. gemmifera被指定为pGE1和pGE2。这些重复序列的长度为180 bp，并且以头对尾的方式组织。它们与A. thaliana和A. arenosa。A. gemmifera和A.川崎树具有pAa、pAgel和pAge2重复序列家族。不同的着丝粒重复序列的序列比较显示，这些家庭共享一个高度保守的区域约50 bp。在四个重复家族中的每一个家族中，两个或三个区域显示出低水平的序列变异。核苷酸序列的平均差异约为10%的家庭和家庭之间的30%，这导致家庭之间的系统发育分析的明确区分。FISH分析表明，pAa、pAgel和pA2家族在A. gemmifera和A.川崎那。在A. gemmifera，A.川崎，两个重复的家庭在场。在A. gemmifera和A. Kawasakiana指出，着丝粒重复序列的均一化的第一步发生在染色体水平。
Centromeric repetitive sequences were isolated from Arabidopsis halleri ssp. gemmifera and A. lyrata ssp. kawasakiana. Two novel repeat families isolated from A. gemmifera were designated pAge1 and pAge2. These repeats are 180 bp in length and are organized in a head-to-tail manner. They are similar to the pAL1 repeats of A. thaliana and the pAa units of A. arenosa. Both A. gemmifera and A. kawasakiana possess the pAa, pAgel and pAge2 repeat families. Sequence comparisons of different centromeric repeats revealed that these families share a highly conserved region of approximately 50 bp. Within each of the four repeat families, two or three regions showed low levels of sequence variation. The average difference in nucleotide sequence was approximately 10% within families and 30% between families, which resulted in clear distinctions between families upon phylogenetic analysis. FISH analysis revealed that the localization patterns for the pAa, pAgel and pAge2 families were chromosome specific in A. gemmifera and A. kawasakiana. In one pair of chromosomes in A. gemmifera, and three pairs of chromosomes in A. kawasakiana, two repeat families were present. The presence of three families of centromeric repeats in A. gemmifera and A. kawasakiana indicates that the first step toward homogenization of centromeric repeats occurred at the chromosome level.