DYNAMIC AND STABLE-POPULATIONS OF MICROTUBULES IN CELLS
DYNAMIC AND STABLE-POPULATIONS OF MICROTUBULES IN CELLS
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DOI：
10.1083/jcb.104.2.277
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发表时间：
1987-02-01
影响因子：
7.8
通讯作者：
KIRSCHNER, M
中科院分区：
文献类型：
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作者：
SCHULZE, E;KIRSCHNER, M
使用一种新的免疫细胞化学技术，我们已经可视化了那些在显微注射后对生物素-微管掺入稳定的细胞中微管的空间排列。在注射后不同时间固定的细胞暴露于生物素标记的微管抗体和几层二次抗体；这些二次抗体层阻止含有生物素的微管与抗微管抗体的反应。没有加入生物素-微管蛋白的微管可以用抗微管蛋白和荧光二抗进行染色。在BSC1细胞中，细胞内的大部分微管以10min的半衰期交换。使用上述技术可以检测到单独的微管群体，这些微管可以稳定地交换1小时或更长时间；与大多数动态微管相比，这些微管具有特征的胚珠周体周围的空间排列。与动态微管不同，大多数稳定的微管是不生长的。平均每个BSC-1单元包含.apprx。700个微管：.apprx。500以4µm的最小速度增长-1100，以.apprx的速度收缩。20µm min-1和.apprx。100只相对更稳定的交易所。讨论了这些稳定的微管的潜在意义。
Using a new immunocytochemical technique, we have visualized the spatial arrangement of those microtubules in cells that are stable to biotin-tubulin incorporation after microinjection. Cells fixed at various periods of time after injection were exposed to antibody to biotinylated tubulin and several layers of secondary antibodies; these layers prevented reaction of biotin-containing microtubules with antitubulin antibodies. The microtubules that had not incorporated biotin-tubulin could then be stained with anti-tubulin and a fluorescent secondary antibody. In BSC1 cells, most microtubules in the cell exchange with a half-time of 10 min. A separate population of microtubules can be detected, using the above techniques, that are stable to exchange for 1 h or more; these have a characteristic pericentrosomal spatial arrangement as compared to the majority of dynamic microtubules. Unlike the dynamic microtubules, most of the stable microtubules are nongrowing. The average BSC-1 cell contains .apprx. 700 microtubules: .apprx. 500 growing at 4 .mu.m min-1, 100 shrinking at .apprx. 20 .mu.m min-1, and .apprx. 100 that are relatively more stable to exchange. The potential significance of these stable microtubules is discussed.