Development of PCR-based assays for the detection of two human mollicute species, Mycoplasma penetrans and M. hominis.
Development of PCR-based assays for the detection of two human mollicute species, Mycoplasma penetrans and M. hominis.
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开发基于 PCR 的检测方法，用于检测两种人类软体动物：穿透支原体和人型支原体。
DOI：
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发表时间：
1994
影响因子：
3.3
通讯作者：
Luc Montagnier
中科院分区：
文献类型：
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作者：
Odile Grau;Rémi Kovacic;Rémi Griffais;Valérie Launay;Luc Montagnier
近年来，基于16SrDNA的聚合酶链反应（PCR）技术被发展用于梨支原体的选择性和敏感性检测。在本研究中，同样的程序，以选择性地检测两个人支原体，M。hominis和M.反式，即使在人类DNA的背景下也具有高水平的灵敏度。对于每种试验，通过检测其他柔膜菌属（包括与相应支原体密切相关的柔膜菌属）、遗传学上接近柔膜菌属的细菌、大肠埃希菌和人外周血单核细胞（PBMC）的DNA，验证了专属性。每种检测都被证明是高度敏感的，因为它可靠地检测到10个DNA分子，即使在人类DNA的背景下。本研究的结果表明，我们的程序的适用性，使用引物设计的PCR检测人柔膜菌具有高特异性和低和可重复的灵敏度阈值。
Recently, a 16S rDNA-based polymerase chain reaction (PCR) assay was developed for the selective and sensitive detection of Mycoplasma pirum. In this study, the same procedure was used in order to selectively detect by PCR two human mycoplasmas, M. hominis and M. penetrans, with a high level of sensitivity even in a context of human DNA. For each assay, the specificity was verified by testing DNA from other mollicute species (including those closely related to the corresponding mycoplasma), from bacteria phylogenetically close to mollicutes, from Escherichia coli and from human peripheral blood mononuclear cells (PBMCs). Each assay proved to be highly sensitive since it reliably detected 10 DNA molecules, even in a context of human DNA. The results of this study demonstrate the suitability of our procedure using primers which were designed for the PCR detection of human mollicutes with a high specificity and a low and reproducible threshold of sensitivity.