DNA duplex-quadruplex exchange as the basis for a nanomolecular machine
DNA duplex-quadruplex exchange as the basis for a nanomolecular machine
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DOI：
10.1073/pnas.0335459100
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发表时间：
2003-02-18
影响因子：
11.1
通讯作者：
Mergny, JL
中科院分区：
文献类型：
--
作者：
Alberti, P;Mergny, JL
目前有很大的兴趣在设计的纳米器件，能够执行线性或旋转运动。蛋白质分子机器在生物学中是丰富的，但最近有人提出，核酸也可以作为模型系统中的纳米分子机器。DNA机器有几种类型的运动：旋转和“剪刀般”的打开和关闭。在这里，我们展示了一种能够进行伸缩运动的纳米机器。这里描述的简单而稳健的装置由单个21个碱基的微粒组成，并且依赖于双链体-四链体平衡，该平衡可以通过顺序添加DNA单链来提供动力，从而产生作为副产物的DNA双链体。两个定义良好的拓扑状态之间的相互转换诱导了5纳米的两冲程，线性电机型的运动，这是由荧光共振能量转移光谱检测。
There is currently great interest in the design of nanodevices that are capable of performing linear or rotary movements. Protein molecular machines are abundant in biology but it has recently been proposed that nucleic acids could also act as nanomolecular machines in model systems. Several types of movements have been described with DNA machines: rotation and "scissors-like" opening and closing. Here we show a nanomachine that is capable of an extension-contraction movement. The simple and robust device described here is composed of a single 21-base oligonucleoticle and relies on a duplex-quadruplex equilibrium that may be fueled by the sequential addition of DNA single strands, generating a DNA duplex as a by-product. The interconversion between two well defined topological states induces a 5-nm two-stroke, linear motor-type movement, which is detected by fluorescence resonance energy transfer spectroscopy.