Differential distribution of gelatinases and tissue inhibitor of metalloproteinase-1 in the rat ovary
Differential distribution of gelatinases and tissue inhibitor of metalloproteinase-1 in the rat ovary
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DOI：
10.1677/joe.0.1580221
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发表时间：
1998-08-01
影响因子：
4
通讯作者：
Bagavandoss, P
中科院分区：
文献类型：
--
作者：
Bagavandoss, P
通过免疫荧光显微镜研究了明胶酶/基质金属蛋白酶-2和-9（MMP-2和MMP-9）以及金属蛋白酶组织抑制剂-1（TIMP-1）在新生儿和促性腺激素引发的未成熟大鼠卵巢中的分布。未成熟雌性Long-Evans大鼠用100μl PBS中的15IU怀孕马血清促性腺激素(PMSG)进行引发。两天后，为诱导排卵，给大鼠注射人绒毛膜促性腺激素（hCG，5 IU/100μl PBS）。在适当的时间处死动物，取出卵巢并进行低温恒温器或石蜡切片处理。还从7天大的新生大鼠中获取卵巢并按上述方法进行处理。在新生大鼠卵巢中，MMP-2 存在于卵泡和卵巢表面上皮中。在新生儿卵巢中未检测到 MMP-9。 TIMP-1 存在于卵母细胞和表面上皮中。在 PMSG 引发的卵巢中，MMP-2 存在于卵巢的颗粒细胞和鞘细胞中。然而，MMP-9 的分布仅限于间质和膜细胞。 TIMP-1主要存在于血管和膜细胞中，颗粒细胞中有少量染色。在发育中的黄体中，黄体细胞和内皮细胞的 MMP-2 呈阳性。 MMP-9 定位仅限于黄体细胞和间质细胞的质膜。 TIMP-1 在黄体毛细血管中清晰可见，在黄体细胞质膜中也有较小程度的观察到。 MMP-2、MMP-9 和 TIMP-1 在黄体中的这种分布在黄体的整个生命周期中持续存在。明胶酶和 TIMP-1 的空间和时间分布表明这些蛋白质在大鼠卵巢中具有独特的作用。
The distribution of gelatinases/matrix metalloproteinases-2 and -9 (MMP-2 and MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) ill neonatal and gonadotropin-primed immature rat ovaries was studied by immunofluorescent microscopy. Immature female Long-Evans rats were primed with 15 IU pregnant mare's serum gonadotropin (PMSG) in 100 mu l PBS. Two days later, to induce ovulation, the rats were injected with human chorionic gonadotropin (hCG, 5 IU/100 mu l PBS). The animals were killed at appropriate times and the ovaries removed and processed for cryostat or paraffin sectioning. Ovaries were also obtained from 7-day-old neonatal rats and processed as above. In the neonatal rat ovary, MMP-2 was present in the follicle and in the ovarian surface epithelium. MMP-9 was not detectable in the neonatal ovary. TIMP-1 was present in the oocyte and in the surface epithelium. In the PMSG-primed ovary, MMP-2 was present in the granulosa and thecal cells of the ovary. MMP-9 distribution, however, was restricted to the interstitial and thecal cells. TIMP-1 was mainly present in the blood vessels and thecal cells, with minor staining in the granulosa cells. In the developing corpus luteum, luteal and endothelial cells were positive for MMP-2. MMP-9 localization was restricted to the plasma membrane of the luteal and interstitial cells. TIMP-1 was clearly observed in the luteal capillaries and, to a lesser extent, in the luteal cell plasma membrane. This distribution of MMP-2, MMP-9, and TIMP-1 in the corpus luteum persisted throughout the life span of the corpus luteum. The spatial and temporal distribution of the gelatinases and TIMP-1 suggests unique roles for these proteins in the rat ovary.