Avian paramyxovirus serotype-1 isolation from migratory birds and environmental water in southern Japan: An epidemiological survey during the 2018/19-2021/2022 winter seasons
Avian paramyxovirus serotype-1 isolation from migratory birds and environmental water in southern Japan: An epidemiological survey during the 2018/19-2021/2022 winter seasons
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DOI：
10.1111/1348-0421.13053
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发表时间：
2023-01-27
影响因子：
2.6
通讯作者：
Fujimoto，Yoshikazu
中科院分区：
文献类型：
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作者：
Matsunaga，Nonoka;Ijiri，Moe;Fujimoto，Yoshikazu
新城疫是由禽副粘病毒1型高致病性病毒(APMV-1)引起的一种高度接触性禽病。尽管在20世纪50年代至21世纪头十年间，日本曾发生过大规模的新城疫疫情，但自2010年以来，任何地方都没有爆发过新城疫。此外，近10年来，日本没有野生鸟类中APMV-1的流行病学调查报告。我们于2018年冬季至2022年在日本南部鹿儿岛县出水平原进行了首次APMV-1流行病学调查。共分离到15株APMV-1，从栖息水和鸭粪中的分离率分别为2.51%和0.10%。这些结果表明，从环境水中分离APMV-1的方法可能是有效监测野生鸟类APMV-1的有效方法。此外，这是首次从环境水样中成功分离到APMV-1的报告。对Fusion(F)基因的遗传分析表明，所有APMV-1分离物都与远东国家水禽之间流行的病毒株密切相关。所有分离株在F基因的裂解部位都有无毒基序，都被认为是家禽中的低致病性病毒。然而，鸡胚蛋的致病性试验表明，无论接种病毒剂量(102-10650%鸡蛋感染剂量)，有些分离物都能杀死所有的鸡胚。这些结果表明，对鸡具有潜在致病性的APMV-1毒株是通过迁徙的野鸟持续带入泉平原的。
Newcastle disease caused by highly pathogenic viruses of avian paramyxovirus serotype‐1 (APMV‐1) is a highly contagious poultry disease. Although a large‐scale epidemic of Newcastle disease had occurred in Japan between the 1950s and the 2000s, there have been no outbreaks anywhere since 2010. In addition, there are no reports of epidemiological surveys of APMV‐1 in wild birds in Japan in the last 10 years. We conducted the first epidemiological survey of APMV‐1 in the Izumi plain, Kagoshima prefecture of southern Japan from the winter of 2018 to 2022. A total of 15 APMV‐1 strains were isolated, and isolation rates from roosting water and duck fecal samples were 2.51% and 0.10%, respectively. These results indicate that the isolation method from environmental water may be useful for efficient surveillance of APMV‐1 in wild birds. Furthermore, this is the first report on the success of APMV‐1 isolation from environmental water samples. Genetic analysis of the Fusion (F) gene showed that all APMV‐1 isolates were closely related to virus strains circulating among waterfowl in Far East Asian countries. All isolates have avirulent motifs in their cleavage site of F genes, all of which were presumed to be low pathogenic viruses in poultry. However, pathogenicity test using embryonated chicken eggs demonstrated that some isolates killed all chicken embryos regardless of viral doses inoculated (102–10650% egg infectious dose). These results indicated that APMV‐1 strains, which are potentially pathogenic to chickens, are continuously brought into the Izumi plain by migrating wild birds.