COMMITMENT OF CELL FATE IN THE EARLY ZEBRAFISH EMBRYO
COMMITMENT OF CELL FATE IN THE EARLY ZEBRAFISH EMBRYO
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DOI：
10.1126/science.8316841
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发表时间：
1993-07-02
期刊：
影响因子：
56.9
通讯作者：
KIMMEL, CB
中科院分区：
文献类型：
--
作者：
HO, RK;KIMMEL, CB
早期斑马鱼胚胎中的单个细胞何时变得不可逆地致力于特定的命运？谱系追踪和命运定位的工作表明，胚盘的边缘细胞产生下胚层衍生的命运（中胚层和内胚层）。然而，这里描述的实验表明，这些边缘胚盘细胞保持多能性，并在整个囊胚晚期和早期原肠胚阶段未定型。在原肠胚形成中期，胚胎细胞被定型为下胚层来源的命运。延时摄影分析显示，定向细胞，当移植异位和异时，可以迁移沿着非典型途径，重新定位自己在一个更正确的环境。
When do single cells in the early zebrafish embryo become irreversibly committed to a specific fate? Work with lineage tracing and fate mapping has shown that the marginal cells of the blastoderm give rise to hypoblast-derived fates (mesoderm and endoderm). However, experiments described here show that these marginal blastoderm cells remain pluripotent and uncommitted throughout the late blastula and early gastrula stages. Embryonic cells become committed to a hypoblast-derived fate at mid-gastrulation. Time-lapse photographic analysis reveals that committed cells, when transplanted heterotopically and heterochronically, can migrate along atypical pathways to reposition themselves within a more correct environment.