Structural basis for docking of peroxisomal membrane protein carrier Pex19p onto its receptor Pex3p
Structural basis for docking of peroxisomal membrane protein carrier Pex19p onto its receptor Pex3p
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DOI：
10.1038/emboj.2010.293
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发表时间：
2010-12-15
期刊：
影响因子：
11.4
通讯作者：
Kato, Hiroaki
中科院分区：
文献类型：
--
作者：
Sato, Yasuhiko;Shibata, Hiroyuki;Kato, Hiroaki
过氧化物体的生物发生需要过氧化物素(Pex)蛋白。Pex3p和Pex19p之间的相互作用对于过氧化体的形成和过氧体膜蛋白(PMPs)的翻译后靶向至关重要。目前尚不清楚Pex3p如何促进Pex19p与Pex19p的特异性相互作用以达到PMP易位的目的。在这里，我们展示了Pex3p的胞液结构域和Pex19p的结合区多肽之间的复合体的三维结构。Pex3p的整体形状是一个长椭圆形，有一个新颖的折叠，即“扭曲的六螺旋束状”。Pex19p结合位点位于Pex3p球体的顶端。Pex19p多肽的16个残基区形成一个a-螺旋并与Pex3p接触；该螺旋在未结合状态下是无序的。Pex19p包含一个特征基序，由亮氨酸三联体(Leu18，Leu21，Leu22)和Phe29组成，它们对Pex3p结合和过氧化酶体的生物发生至关重要。EMBO期刊(2010)29,4083-4093。DOI：10.1038/Intemj.2010.293；2010年11月19日在线发布
Peroxisomes require peroxin (Pex) proteins for their biogenesis. The interaction between Pex3p, which resides on the peroxisomal membrane, and Pex19p, which resides in the cytosol, is crucial for peroxisome formation and the post-translational targeting of peroxisomal membrane proteins (PMPs). It is not known how Pex3p promotes the specific interaction with Pex19p for the purpose of PMP translocation. Here, we present the three-dimensional structure of the complex between a cytosolic domain of Pex3p and the binding-region peptide of Pex19p. The overall shape of Pex3p is a prolate spheroid with a novel fold, the 'twisted six-helix bundle.' The Pex19p-binding site is at an apex of the Pex3p spheroid. A 16-residue region of the Pex19p peptide forms an a-helix and makes a contact with Pex3p; this helix is disordered in the unbound state. The Pex19p peptide contains a characteristic motif, consisting of the leucine triad (Leu18, Leu21, Leu22), and Phe29, which are critical for the Pex3p binding and peroxisome biogenesis. The EMBO Journal (2010) 29, 4083-4093. doi: 10.1038/emboj.2010.293; Published online 19 November 2010