Taurine supplementation does not decrease homocysteine levels and liver injury induced by a choline-deficient diet
Taurine supplementation does not decrease homocysteine levels and liver injury induced by a choline-deficient diet
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DOI：
10.1016/j.lfs.2014.04.015
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发表时间：
2014-06-06
期刊：
影响因子：
6.1
通讯作者：
Jordao, Alceu Afonso
中科院分区：
文献类型：
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作者：
Deminice, Rafael;Rosa, Flavia Troncon;Jordao, Alceu Afonso
目的：为探讨补充牛磺酸对胆碱缺乏饮食大鼠同型半胱氨酸（Hcy）代谢及肝损伤的影响，将30只大鼠随机分为3组（n = 10），分别给予对照组（C组）、胆碱缺乏饮食组（CDD组）和胆碱缺乏饮食+牛磺酸组（CDDT组）。CDD和CDDT由AIN-93组成，不含推荐的2.5%胆碱含量，CDDT通过添加2.5%牛磺酸进行补充。关键发现：4周的英迪娜CDD导致血浆Hcy显著增加（50%），以及肝脏S-腺苷甲硫氨酸（SAM）浓度和S-腺苷甲硫氨酸/S-腺苷同型半胱氨酸比值降低。与对照组相比，未发现血浆蛋氨酸和半胱氨酸血浆水平的变化。灌胃4周后，肝脏总脂肪、丙二醛（MDA）和血浆丙氨酸氨基转移酶（ALT）水平显著升高（P < 0.05）。此外，与对照组相比，在喂食CDD的大鼠中发现了还原型肝脏谷胱甘肽（GSH）水平和还原型/氧化型谷胱甘肽比值（GSH/GSSG）。牛磺酸补充CDD使参与再甲基化途径的基因BHMT和CHDH正常化，这些基因单独受到CDD的损害。然而，牛磺酸补充未能防止CD诱导的Hcy代谢紊乱和肝损伤。此外，牛磺酸添加到CDD导致PEMT，CHKa，和CHKb，参与磷脂酰胆碱（PC）合成和肝脏脂肪accumulation.Significance的关键基因的表达下降：牛磺酸补充未能改善受损的Hcy代谢和CDD摄入引起的肝损伤。(C)2014爱思唯尔公司All rights reserved.
Aims: The aim of this study is to examine the effects of taurine supplementation on homocysteine (Hcy) metabolism and liver injury in rats fed a choline-deficient diet.Main methods: Thirty rats were divided into three groups (n = 10), to receive one of the following diets for 4 weeks: control diet (C), choline-deficient diet (CDD), or choline-deficient diet supplemented with taurine (CDDT). The CDD and the CDDT consisted of AIN-93 without the recommended choline content of 2.5%, and the CDDT was supplemented by the addition of 2.5% taurine.Key findings: Four weeks of ingesting a CDD resulted in a significant increase in plasma Hcy (50%) as well as a decrease in liver S-adenosylmethionine (SAM) concentration and S-adenosylmethionine/S-adenosylhomocysteine ratio. No changes were found in plasma methionine and cysteine plasma levels compared to control group. Four weeks of ingesting a CDD also caused a significant (P < 0.05) increase in hepatic total fat, hepatic malondialdehyde (MDA), and plasma alanine aminotransferase (ALT) levels. In addition, reduced hepatic glutathione (GSH) levels and reduced/oxidized glutathione ratios (GSH/GSSG) were found in rats fed a CDD compared to controls. Taurine supplementation of the CDD normalized genes involved in the remethylation pathway, BHMT and CHDH, which were impaired by CDD alone. However, taurine supplementation failed to prevent CDD-induced Hcy metabolism disturbances and hepatic injury. Also, taurine added to CDD caused decreased expression of PEMT, CHKa, and CHKb, key genes involved in phosphatidylcholine (PC) synthesis and liver fat accumulation.Significance: Taurine supplementation failed to ameliorate impaired Hcy metabolism and liver injury caused by CDD intake. (C) 2014 Elsevier Inc. All rights reserved.