Copper (II) ions potently inhibit purified PrPres amplification.
Copper (II) ions potently inhibit purified PrPres amplification.
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铜 (II) 离子可有效抑制纯化的 PrPres 扩增。
DOI：
10.1111/j.1471-4159.2006.03650.x
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发表时间：
2006
影响因子：
4.7
通讯作者：
Supattapone,Surachai
中科院分区：
文献类型：
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作者：
Orem,NicholasR;Geoghegan,JamesC;Deleault,NathanR;Kascsak,Richard;Supattapone,Surachai
The structural conversion of a host protein, PrPC, into a protease‐resistant isoform, PrPres, is the central event in the pathogenesis of infectious prion diseases. Purification of native PrPCmolecules from hamster brain by either cation exchange or immobilized chelator chromatographic resins yielded preparations that supported efficient amplification of scrapie‐induced PrPresin vitro. Using these purified preparations, we determined thatin vitroPrPres amplification was inhibited by CuCl2and ZnCl2at IC50concentrations of ∼400 nmand 10 μm, respectively. In contrast, 100 μmMnCl2did not directly inhibit PrPres amplification or block Cu2+‐mediated inhibition. Additionally, the inhibition of PrPres amplification by Cu2+ions could be reversed by addition of either neocuproine or imidazole. Cu2+inhibited PrPres amplification in both the presence and absence of stimulatory polyanion molecules. These biochemical findings support the hypothesis that Cu2+ions might regulate the pathogenesis of prion diseasesin vivo.
美国植入人工晶状体的最新情况。
DOI：
--
发表时间：
1984
期刊：
American journal of ophthalmology-glaucoma
影响因子：
--
作者：
W. Stark;A. C. Terry;D. Worthen;G. Murray
通讯作者：
G. Murray
白内障手术：随机对照试验的中期结果和并发症。
DOI：
10.1136/bjo.70.6.402
发表时间：
1986
期刊：
The British journal of ophthalmology
影响因子：
--
作者：
通讯作者：
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