Use of an HPLC photodiode-array detector in a study of the nature of a black tea liquor
Use of an HPLC photodiode-array detector in a study of the nature of a black tea liquor
复制标题
使用 HPLC 光电二极管阵列检测器研究红茶酒的性质
DOI：
--
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
H. Nursten
中科院分区：
文献类型：
--
作者：
R. G. Bailey;I. McDowell;H. Nursten
非挥发性，水溶性成分的红茶进行了研究，通过使用反相高效液相色谱法与光电二极管阵列检测器，使用线性梯度为此目的而开发。该检测器从一次注入的红茶液体中产生的数据允许获得有关成分的性质和纯度的信息。通过光谱数据和参比化合物给出的保留时间鉴定了以下成分：咖啡因、可可碱、（−）-表儿茶素没食子酸酯、（−）-表没食子儿茶素没食子酸酯、没食子酸、绿原酸（5-O-咖啡酰奎尼酸）、对香豆酰奎尼酸、槲皮素3-葡萄糖苷、槲皮素3-鼠李糖基葡萄糖苷、山奈酚3-鼠李糖基葡萄糖苷和茶黄素二没食子酸酯。通过光谱数据对其他峰进行分类，但如果没有参比化合物，则无法进行结论性归属。最后，一组峰可以被归类为有色酚类色素，并暂时指定为茶红素。
The non-volatile, water-soluble constituents of black tea were studied by using reversed-phase HPLC with a photodiode-array detector, using a linear gradient developed for this purpose. The data generated by this detector from one injection of a black tea liquor allowed information on the nature and purity of the constituents to be obtained. The following constituents were identified by the spectral data and the retention times given by reference compounds: caffeine, theobromine, (−)-epicatechin gallate, (−)- epigallocatechin gallate, gallic acid, chlorogenic acid (5-O-caffeoylquinic acid), p-coumaroylquinic acid, quercetin 3-glucoside, quercetin 3-rhamnosylglucoside, kaempferol 3-rhamnosylglucoside, and theaflavin digallate. Other peaks were classified by spectral data, but without reference compounds conclusive assignments could not be made. Finally, a group of peaks could be classified as coloured phenolic pigments, and tentatively assigned as thearubigins.