A pilot-scale study on start-up and stable operation of mainstream partial nitrification-anammox biofilter process based on online pH-DO linkage control
A pilot-scale study on start-up and stable operation of mainstream partial nitrification-anammox biofilter process based on online pH-DO linkage control
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DOI：
10.1016/j.cej.2018.06.007
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发表时间：
2018-10-15
影响因子：
15.1
通讯作者：
Wang, Hongchen
中科院分区：
文献类型：
--
作者：
Jiang, Hangcheng;Liu, Guo-hua;Wang, Hongchen
对主流部分硝化-厌氧氨氧化生物滤池（PN/AF）系统启动及稳定运行进行了中试研究。部分硝化系统基于在线pH-溶解氧(DO)联动控制策略对亚硝酸氧化菌(NOB)进行淘汰。固定化厌氧氨氧化系统是一种上传生物过滤反应器，其中厌氧氨氧化细菌被固定在火山载体上。结果表明，部分硝化系统出水亚硝酸盐积累率（NAR）保持在90%以上，NO2-N/NH4+-N值保持在0.96+/-0.18；整个系统稳定运行后，出水氨氮平均浓度低于5 mg/L，总无机氮（TIN）去除负荷可达到0.11 kg/m(3).d。高通量测序结果显示，运行30天后，NOB（主要是Nitrospira、Nitrotoga）脱离部分硝化系统，从Krona图谱上观察到其相对丰度不足1%，氨氧化菌（AOB，主要是Nitrosomonas）成为硝化细菌的优势种，AOB最终稳定在8%。优势厌氧氨氧化菌被鉴定为Candidatus Brocadia属，并在火山生物滤池反应器的下侧发现了一种罕见的厌氧氨氧化菌Asahi BRW_2。可以发现，在线pH-DO联动控制策略使得NOB从主流SBR系统中脱颖而出，实现了稳定的部分硝化。此外，高 DO (2.5 +/- 0.5 mg/L) 条件有利于 NOB 的淘汰和亚硝化的稳定。厌氧氨氧化菌可以很好地固定在火山载体上，具有较低的耐氨能力。该研究有助于主流厌氧氨氧化工艺的工程应用。
A pilot-scale study on start-up and stable operation of mainstream partial nitrification-anammox biofilter (PN/AF) system was conducted. The partial nitrification system was controlled based on online pH-dissolved oxygen (DO) linkage control strategy for nitrite oxidizing bacteria (NOB) out-selection. The immobilized anammox system was an upload biofilter reactor where anammox bacteria were immobilized on the volcanic carriers. The results showed that the nitrite accumulation ratio (NAR) was maintained above 90%, and the NO2-N/NH4+-N value was kept at 0.96 +/- 0.18 in the effluent of the partial nitrification system; the mean ammonia concentration of the effluent was below 5 mg/L, and total inorganic nitrogen (TIN) removal loading could achieve 0.11 kg/m(3).d after the stable operation in the whole system. The results of high-throughout sequencing showed that the NOB (mainly Nitrospira, Nitrotoga) was out of the partial nitrification system after 30-day operation, the relative abundance of which is less than 1% observed from Krona maps, and ammonia oxidizing bacteria (AOB, mainly Nitrosomonas) became the dominant species of nitrobacteria, the 8% of AOB stabilized finally. Dominant anammox bacteria was identified as genus Candidatus Brocadia, and one kind of rare anammox named Asahi BRW_2 was found to be at the downside of the volcanic biofilter reactor. It could be found the online pH-DO linkage control strategy allowed out-selection of NOB from the mainstream SBR system, and realized a stable partial nitrification. In addition, high DO (2.5 +/- 0.5 mg/L) condition was found to be favorable for NOB out-selection and the stabilization of nitritation. The anammox bacteria could be well immobilized in volcanic carriers, with an ability of low ammonia resistance. The study was helpful for engineering application of mainstream anammox process.