Chemical protection and cell-cycle effects on radiation-induced mutagenesis.
Chemical protection and cell-cycle effects on radiation-induced mutagenesis.
复制标题
化学保护和细胞周期对辐射诱导突变的影响。
DOI：
10.1111/j.1365-2184.1992.tb01434.x
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发表时间：
1992
影响因子：
8.5
通讯作者：
Sigdestad,CP
中科院分区：
文献类型：
--
作者：
Grdina,DJ;Sigdestad,CP
收获对数生长期的中国仓鼠卵巢细胞，并通过离心淘洗方法分离成富含高达95％G1期、70％S期和65％G2+M期细胞的亚群。通过流式细胞术常规监测细胞周期分布。淘洗后，将每个富集细胞组分的细胞等分试样在存在或不存在 4 MM 2-[(氨丙基)氨基]乙硫醇 (WR-1065) 的情况下在 37°C 下孵育 30 分钟。然后用来自 JANUS 研究反应堆的 60 Co γ 射线或裂变谱中子照射细胞。测定了细胞杀伤和诱变。无论使用何种辐射质量，富集于 G1 期的细胞对次黄嘌呤鸟嘌呤磷酸核糖基转移酶位点的辐射诱导突变最敏感。对于每个淘析器分离的细胞群，WR-1065 所施加的相对保护程度有所不同。然而，无论使用的辐射质量或测试的生物终点如何，在富含 G1 的人群中都观察到了最大程度的保护。
Chinese hamster ovary cells in the exponential phase of growth were harvested and separated by the method of centrifugal elutriation into subpopulations enriched with up to 95% G1phase, 70% S phase and 65% G2+ M phase cells. Cell cycle distributions were routinely monitored by flow cytometry. Following elutriation, aliquots of cells from each of the enriched cell fractions were incubated in the presence or absence of 4 MM of 2–[(aminopropyl)amino] ethanethiol (WR‐1065) for 30 min at 37°C. The cells were then irradiated with60Co γ‐rays or fission‐spectrum neutrons from the JANUS research reactor. Both cell killing and mutagenesis were determined. Regardless of the radiation quality used, cells enriched in G1phase were the most sensitive to radiation‐induced mutagenesis at the hypoxanthine‐guanine phos‐phoribosyl transferase locus. The relative magnitude of protection exerted by WR‐1065 differed for each of the elutriator separated cell populations. The greatest magnitude of protection, however, was observed for G1‐enriched populations, regardless of the radiation quality used or the biological end‐point tested.