Physical and Electrochemical Properties of PEDOT:PSS as a Tool for Controlling Cell Growth
Physical and Electrochemical Properties of PEDOT:PSS as a Tool for Controlling Cell Growth
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DOI：
10.1021/acsami.5b04768
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发表时间：
2015-08-19
影响因子：
9.5
通讯作者：
Fraboni, Beatrice
中科院分区：
文献类型：
--
作者：
Marzocchi, Marco;Gualandi, Isacco;Fraboni, Beatrice
导电聚合物是组织工程应用中很有前途的材料，因为它们既可以为活细胞的物理支持提供生物相容性支架，又可以通过其导电性和可逆掺杂传输电和机械刺激。在这项工作中，利用自旋涂层和电化学聚合两种不同的技术制备了生物电子学应用中最有前途的材料之一聚（3,4-乙烯二氧噻吩）聚苯乙烯磺酸盐（PEDOT:PSS）薄膜，并随后通过外部偏压的应用改变了它们的氧化态。通过循环伏安法和分光光度法研究了这些不同类型的PEDOT:PSS的电化学性能，以评估氧化过程的有效性及其随时间的稳定性。利用原子力显微镜（AFM）、水接触角测量和薄片电阻测量研究了它们的表面物理性质及其对PEDOT:PSS氧化还原态的依赖。最后，将人胶质母细胞瘤多形性细胞（T98G）和原代人真皮成纤维细胞（hDF）培养在不同氧化状态的PEDOT:PSS膜上，发现底物对细胞生长速率的影响具有很强的细胞依赖性：还原样品促进了T98G的生长，而hDF仅在与细胞培养基具有强化学相互作用的氧化底物上生长更有效。
Conducting polymers are promising materials for tissue engineering applications, since they can both provide a bio compatible scaffold for physical support of living cells, and transmit electrical and mechanical stimuli thanks to their electrical conductivity and reversible doping. In this work, thin films of one of the most promising materials for bioelectronics applications, poly(3,4-ethylenedioxythiophene) poly(styrenesulfonate) (PEDOT:PSS), are prepared using two different techniques, spin coating and electrochemical polymerization, and their oxidation state is subsequently changed electrochemically with the application of an external bias. The electrochemical properties of these different types of PEDOT:PSS are studied through cyclic voltammetry and spectrophotometry to assess the effectiveness of the oxidation process and its stability over time. Their surface physical properties and their dependence on the redox state of PEDOT:PSS are investigated using atomic force microscopy (AFM), water contact angle goniometry and sheet resistance measurements. Finally, human glioblastoma multiforme cells (T98G) and primary human dermal fibroblasts (hDF) are cultured on PEDOT:PSS films with different oxidation states, finding that the effect of the substrate on the cell growth rate is strongly cell-dependent: T98G growth is enhanced by the reduced samples, while hDF growth is more effective only on the oxidized substrates that show a strong chemical interaction with the cell culture medium.