Osteoclast 121F antigen expression during osteoblast conditioned medium induction of osteoclast-like cells in vitro: relationship to calcitonin responsiveness, tartrate resistant acid phosphatase levels, and bone resorptive activity.
Osteoclast 121F antigen expression during osteoblast conditioned medium induction of osteoclast-like cells in vitro: relationship to calcitonin responsiveness, tartrate resistant acid phosphatase levels, and bone resorptive activity.
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破骨细胞121F抗原表达在成骨细胞条件培养基体外诱导破骨细胞样细胞过程中：与降钙素反应性、抗酒石酸酸性磷酸酶水平和骨吸收活性的关系。
DOI：
10.1002/jbmr.5650100109
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Osdoby,P
中科院分区：
文献类型：
--
作者：
Collin-Osdoby,P;Oursler,MJ;Rothe,L;Webber,D;Anderson,F;Osdoby,P
破骨细胞从造血前体分化为多核细胞，独特地能够去除骨基质的有机和无机成分，这是一个多步骤的过程，在此过程中，破骨细胞获得骨吸收活性和生理调节所必需的专门特性。在这些特征中，有一种新型质膜糖蛋白，可与抗破骨细胞单克隆抗体121 F反应，在破骨细胞分化过程中表达，与Mn 2 +/Fe 2+超氧化物歧化酶具有结构和功能同源性，并被假设可保护破骨细胞免受活性骨吸收过程中产生的超氧化物自由基的破坏作用。我们以前曾报道过，这种膜抗原的表达诱导多核巨细胞时，丰富的骨髓单个核细胞培养在条件培养基从禽颅骨。本文报告的研究旨在研究121 F抗体反应性破骨细胞膜抗原的表达与抗酒石酸酸性磷酸酶水平、骨吸收活性、降钙素反应性和在存在或不存在可扩散成骨细胞分泌因子的情况下形成的禽骨髓源性多核巨细胞的超微结构特征之间的关系。对从相同动物分离的体内形成的破骨细胞进行平行分析以进行直接比较。在本报告中，我们展示了：（1）121 F单克隆抗体反应性破骨细胞膜抗原在巨细胞中由可溶性成骨细胞衍生因子以物种不受限制但浓度和时间依赖性的方式稳定诱导;（2）成骨细胞介导的抗原诱导反映在与121 F抗体反应的细胞数量增加和单个细胞表达升高，如通过ELISA和组织形态计量学测定的;（3）成骨细胞条件培养基除了在骨髓细胞中诱导该抗原外，还提高了这些禽类巨细胞中的其它确定性破骨细胞特征，包括它们的TRAP活性、响应降钙素的细胞从骨表面回缩、骨吸收功能和一系列其它破骨细胞抗原标志物的表达;和（4）单独分泌的成骨细胞产物不能将体外形成的骨髓巨细胞的这些特征的水平提高到与体内形成的破骨细胞相关的程度。因此，单独的成骨细胞可溶性因子似乎不能促进骨髓细胞在体外完全分化为成熟的骨吸收破骨细胞。这种诱导骨髓模型系统，结合监测特异性单克隆抗体提供的骨细胞标志物的扩展谱的能力，因此可以作为一种有价值的工具，用于研究破骨细胞细胞分化的中间阶段，并用于识别负责其部分或完全发育成独特的骨吸收细胞的信号。
Osteoclast differentiation from hematopoietic precursors into multinucleated cells uniquely capable of removing the organic and inorganic components of bone matrix occurs in a multistep process, during which osteoclasts acquire the specialized characteristics necessary for bone resorptive activity and physiological regulation. Among those traits is a novel plasma membrane glycoprotein, reactive with the anti‐osteoclast monoclonal antibody 121F, which is expressed during the course of osteoclast differentiation, shares structural and functional homologies with Mn2+/Fe2+superoxide dismutase, and has been hypothesized to protect the osteoclast from the damaging effects of superoxide radicals generated during active bone resorption. We have reported previously that the expression of this membrane antigen is induced on multinucleated giant cells when the profusion marrow mononuclear cells are cultured in conditioned medium from avian calvaria. The studies reported here were designed to investigate the relationship between expression of the 121F antibody‐reactive osteoclast membrane antigen and tartrate resistant acid phosphatase levels, bone resorptive activity, calcitonin responsiveness, and ultrastructural features of avian bone marrow‐derived multinucleated giant cells formed either in the presence or absence of diffusible osteoblast secreted factors. Parallel analyses of in vivo formed osteoclasts isolated from the same animals were performed for direct comparisons. In this report we demonstrate: (1) that the 121F monoclonal antibody‐reactive osteoclast membrane antigen is stably induced in giant cells by soluble osteoblast‐derived factors in a species nonrestricted but concentration‐ and temporal‐dependent manner; (2) that osteoblast‐mediated antigen induction is reflected in both increased numbers of cells and elevated expression of individual cells that are reactive with the 121F antibody, as determined by ELISA and histomorphometry; (3) that osteoblast conditioned medium, in addition to inducing this antigen in bone marrow cells, also elevates other defining osteoclast characteristics in these avian giant cells including their TRAP activity, cell retraction from the bone surface in response to calcitonin, bone resorptive function, and expression of a series of additional osteoclast antigenic markers; and (4) that secreted osteoblast products alone do not raise the levels of these traits for in vitro formed marrow giant cells to the extent associated with in vivo formed osteoclasts. Therefore, osteoblast soluble factors alone appear unable to promote the full differentiation of bone marrow cells in vitro into mature bone‐resorbing osteoclasts. This inductive bone marrow model system, in conjunction with the ability to monitor an expanded profile of osteoclastic markers afforded by specific monoclonal antibodies, may therefore serve as a valuable tool for investigating intermediate stages of osteoclast cytodifferentiation and for identifying signals responsible for their partial or complete development into unique bone‐resorbing cells.