Role of Chlamydia pneumoniae in community-acquired pneumonia in hospitalized Jordanian adults
Role of Chlamydia pneumoniae in community-acquired pneumonia in hospitalized Jordanian adults
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DOI：
10.3855/jidc.6590
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发表时间：
2016-03-01
影响因子：
1.9
通讯作者：
Al-Younes, Hesham M.
中科院分区：
文献类型：
--
作者：
Al-Aydie, Suzan N.;Obeidat, Nathir M.;Al-Younes, Hesham M.
简介：本研究调查了肺炎衣原体在约旦成人社区获得性肺炎（CAP）病因学中的作用。方法：纳入80例住院CAP患者和110名健康成人。采用微免疫荧光法（MIF）测定血清中肺炎原体IgG抗体的总流行率和急性感染率，滴度分别为1:16和1:512。此外，采用巢式聚合酶链反应（PCR）检测鼻咽标本和血巴菲被标本中的肺炎原体DNA。结果：CAP病例衣原体IgG的总体流行率高于对照组（70%对61.8%）。同样，患者的急性感染率高于对照组（16.3%对5.5%）。通过关注1:16检测呈阳性的受试者，23.2%的CAP病例可检测到急性感染，而血清阳性对照为8.8%。对照组和患者鼻咽标本中分别有8.2%和8.8%的标本检出衣原体DNA。对照组和病例巴菲长毛鼠pcr阳性率分别为10.9%和7.5%。当评估两种检测方法检测病原菌的性能时，MIF和PCR的敏感性较低且具有可比性。然而，MIF比PCR具有更高的特异性、阳性预测值和阴性预测值。结论：基于mif的数据表明，肺炎梭菌可能是约旦CAP的潜在致病因子。本研究可为阐明肺炎梭菌与其他共感染病原体在呼吸道疾病病因学中的确切作用提供依据。
Introduction: This study investigated the role of Chlamydia pneumoniae in the etiology of community-acquired pneumonia (CAP) in Jordanian adults.Methodology: Eighty hospitalized CAP patients and 110 healthy adults were enrolled. Overall prevalences of C. pneumoniae IgG antibodies in sera and the rate of acute infection were estimated, using the microimmunofluorescence method (MIF), at titers of 1: 16 and 1: 512, respectively. Moreover, a nested polymerase chain reaction (PCR) was used to detect C. pneumoniae DNA in nasopharyngeal and blood Buffy coat samples.Results: Overall chlamydial IgG prevalence was higher in CAP cases than controls (70% versus 61.8%). Similarly, higher rate of acute infection was found in patients than in controls (16.3% versus 5.5%). By focusing on subjects testing positive at 1: 16, acute infection was detectable in 23.2% of CAP cases, compared with 8.8% of seropositive controls. Chlamydial DNA was confirmed in 8.2% and 8.8% of nasopharyngeal specimens from controls and patients, respectively. Moreover, 10.9% and 7.5% of Buffy coats from controls and cases, respectively, were PCR-positive. When performances of both assays for detection of the pathogen were assessed, the sensitivities of MIF and PCR were low and comparable. However, MIF demonstrated higher specificity, positive predictive value, and negative predictive value than PCR.Conclusions: MIF-based data indicate that C. pneumoniae could be a potential causal agent of CAP in Jordan. This study may serve as a basis to elucidate the exact role C. pneumoniae and other co-infecting pathogens in the etiology of respiratory tract disease.