Megakaryocytopoiesis in culture: modulation by cholinergic mechanisms.
Megakaryocytopoiesis in culture: modulation by cholinergic mechanisms.
复制标题
培养物中的巨核细胞生成：胆碱能机制的调节。
DOI：
10.1002/jcp.1041030205
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发表时间：
1980
影响因子：
5.6
通讯作者：
Harker,LA
中科院分区：
文献类型：
--
作者：
Burstein,SA;Adamson,JW;Harker,LA
Treatment of murine bone marrow cultures with the cholinergic agonist carbamylcholine enhanced megakaryocytic colony growth by as much as 65%. In contrast, adrenergic agonists had no such effect. Addition to cultures of dibutyryl cyclic GMP (db‐cGMP) also enhanced megakaryocytic colonies up to 50%, whereas dibutyryl cyclic AMP (db‐cAMP) had no effect. Sodium nitroprus‐side and sodium nitrite, putative guanyl cyclase activators, also enhanced colony numbers, as did imidazole, a postulated cGMP phosphodiesterase inhibitor. Preincubation of marrow for two hours with carbamylcholine resulted in both an increase in colony numbers (58%) and percent of progenitors in DNA synthesis (48%, compared to 14% for controls) as determined by tritiated thymidine suicide studies. Treatment of mice with the acetylcholinesterase inhibitor neostigmine resulted in an increase in CFU‐M/humerus (62%) and percent in DNA synthesis (45%). These data indicate that (1) cholinergic, but not adrenergic, agonists modulate megakaryocytopoiesis in culture; (2) this effect may be mediated by cyclic GMP; and (3) only a brief period of exposure of marrow cells to agonist results in enhancement of megakaryocytic colonies.
